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Heavy metals removal from wastewater using extracellular polymeric substances produced by cloacibacterium normanense in wastewater sludge supplemented with crude glycerol and study of extracellular polymeric substances extraction by different methods.

Nouha, Klai; Kumar, Ram Saurabh; Tyagi, Rajeshwar Dayal (2016). Heavy metals removal from wastewater using extracellular polymeric substances produced by cloacibacterium normanense in wastewater sludge supplemented with crude glycerol and study of extracellular polymeric substances extraction by different methods. Bioresource Technology , vol. 212 . p. 120-129. DOI: 10.1016/j.biortech.2016.04.021.

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Résumé

Extracellular polymeric substances synthesis by Cloacibacterium was affected by different concentrations of glycerol in the medium. The concentration of EPS in 72 h fermentation was increased from 13 g/L with no external carbon supplementation to 21.3 ± 0.7 g/L with 2% (w/v) crude glycerol addition. Physical and chemical extraction methods (heating, centrifugation and ethylene diamine tetra-acetic acid (EDTA)) were used in this study and their performance to extract EPS was compared. A significant variation in concentration of extracted B-EPS (broth-EPS) by heating (20.8 ± 0.5 g/L) and centrifugation (21.3 ± 0.7 g/L) extraction methods was not observed. However, in case of extraction with EDTA (5 g/L), the B-EPS concentration extracted was 25.5 ± 0.9 g/L, which exhibited high flocculation activity of 95.3 ± 0.5% at optimum dose of 23.1 mg B-EPS/g Kaolin. Moreover, Ni removal efficiency of 80% from primary treated wastewater was achieved using 35 mg/L of B-EPS extracted by centrifugation method.

Type de document:
Mots-clés libres: wastewater sludge; crude glycerol; extracellular polymeric substance; flocculation activity; heavy metal removal
Centre: Centre Eau Terre Environnement
Date de dépôt: 20 déc. 2016 16:18
Dernière modification: 20 déc. 2016 16:18
URI: http://espace.inrs.ca/id/eprint/4001

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