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Biohydrogen production by co-fermentation of crude glycerol and apple pomace hydrolysate using co-culture of Enterobacter aerogenes and Clostridium butyricum.

Pachapur, Vinayak Laxman; Sarma, Saurabh Jyoti; Brar, Satinder Kaur; Le Bihan, Yann; Buelna, Gerardo; Verma, Mausam P. (2015). Biohydrogen production by co-fermentation of crude glycerol and apple pomace hydrolysate using co-culture of Enterobacter aerogenes and Clostridium butyricum. Bioresource Technology , vol. 193 . p. 297-306. DOI: 10.1016/j.biortech.2015.06.095.

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Résumé

Co-substrate utilization of various wastes with complementary characteristics can provide a complete medium for higher hydrogen production. This study evaluated potential of apple pomace hydrolysate (APH) co-fermented with crude glycerol (CG) for increased H₂ production and decreased by-products formation. The central composite design (CCD) along with response surface methodology (RSM) was used as tool for optimization and 15 g/L of CG, 5 g/L of APH and 15% (v/v) inoculum were found to be optimum to produce as high as 26.07±1.57 mmol H₂/L of medium. The p-value of 0.0017 indicated that APH at lower concentration had a significant effect on H₂ production. By using CG as sole carbon source, reductive pathway of glycerol metabolism was favored with 19.46 mmol H₂/L. However, with APH, oxidative pathway was favored with higher H₂ production (26.01 mmol/L) and decrease in reduced by-products (1,3-propanediol and ethanol) formation. APH inclusion enhanced H₂ production, and decreased substrate inhibition.

Type de document: Article
Mots-clés libres: apple pomace hydrolysate; biohydrogen; co-culture; co-substrate; crude glycerol
Centre: Centre Eau Terre Environnement
Date de dépôt: 23 avr. 2018 17:49
Dernière modification: 23 avr. 2018 17:49
URI: http://espace.inrs.ca/id/eprint/3901

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