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Evidence of metabolic shift on hydrogen, ethanol and 1,3-propanediol production from crude glycerol by nitrogen sparging under micro-aerobic conditions using co-culture of Enterobacter aerogenes and Clostridium butyricum.

Pachapur, Vinayak Laxman; Sarma, Saurabh Jyoti; Brar, Satinder Kaur; Le Bihan, Yann; Buelna, Gerardo; Soccol, Carlos Ricardo (2015). Evidence of metabolic shift on hydrogen, ethanol and 1,3-propanediol production from crude glycerol by nitrogen sparging under micro-aerobic conditions using co-culture of Enterobacter aerogenes and Clostridium butyricum. Science of The Total Environment , vol. 40 , nº 28. p. 8669-8676. DOI: 10.1016/j.ijhydene.2015.05.024.

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Résumé

Hydrogen (H₂), a possible future clean energy carrier, requires process-based improvement routes for cutting down the production cost. The impact of nitrogen (N₂) sparging on H₂ production during co-culture system of Enterobacter aerogenes and Clostridium butyricum from crude glycerol (CG) was studied to reduce the overall process cost. H₂ production using 1% CG under nitrogen sparged medium before autoclaving resulted in 1.2 mmol-H₂/mol of glycerol in comparison to 1.5 mmol-H₂/mol of glycerol without sparging. In the presence of air ranging from 5 mL to 75 mL in the headspace volume, H₂ production increased to a maximum of 26.14 mmol/L with 1.4 g/L of ethanol production. The concentration of 1,3-propanediol with N2 sparging was around 3.0 g/L and decreased to 0.5 g/L due to presence of 75 mL of air in the headspace. This observation can be attributed to a shift from reductive to oxidative metabolism of glycerol. A process-based improvement strategy to optimize H₂ formation resulted in metabolic pathway shift from reductive to oxidative with increase in H₂ production. Synergistic influence of co-culture system in absence of expensive reducing agent and without nitrogen sparging step can offer a better process-based economic strategy for H₂ production, minimize the metabolite production and increase field-scale application of biodiesel plant.

Type de document: Article
Mots-clés libres: co-culture; crude glycerol; ethanol; hydrogen; nitrogen sparging; 1,3-Propanediol
Centre: Centre Eau Terre Environnement
Date de dépôt: 23 avr. 2018 17:46
Dernière modification: 23 avr. 2018 17:46
URI: http://espace.inrs.ca/id/eprint/3900

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