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Production of extracellular polymeric substances (EPS) by Serratia sp.1 using wastewater sludge as raw material and flocculation activity of the EPS produced.

Bezawada, Jyothi; Hoang, N. V.; More, Tanaji; Yan, Song; Tyagi, Neha; Tyagi, Rajeshwar Dayal; Surampalli, Rao Y. (2013). Production of extracellular polymeric substances (EPS) by Serratia sp.1 using wastewater sludge as raw material and flocculation activity of the EPS produced. Journal of Environmental Management , vol. 128 . p. 83-91. DOI: 10.1016/j.jenvman.2013.04.039.

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Résumé

Growth profile and extracellular polymeric substances (EPS) production of Serratia sp.1 was studied in shake flask fermentation for 72h using wastewater sludge as raw material. Maximum cell concentration of 6.7×109cfu/mL was obtained at 48h fermentation time. EPS dry weight, flocculation activity and dewaterability of different EPS (tightly bound or TB-EPS, loosely bound or LB-EPS and broth-EPS or B-EPS) were also measured. The highest concentration of LB-EPS (2.45g/L) and TB-EPS (0.99g/L) were attained at 48h of fermentation. Maximum flocculation activity and dewaterability (δCST) of TB-EPS (76.4%, 14.5s and 76.5%, 15.5s), LB-EPS (67.8%, 8.1s and 64.7%, 7.6s) and broth EPS (61%, 6.1s and 70.4%, 6.8s) were obtained at 36 and 48h of growth. Higher flocculation activity and dewaterability were achieved with TB-EPS than with the two other EPS. Characterization of TB-EPS and LB-EPS was done in terms of their protein and carbohydrate content. Protein content was much higher in TB-EPS where as carbohydrate content was only slightly higher in TB-EPS than LB-EPS. Morphology of the Serratia strain after fermentation in sludge and TSB was observed under a scanning electron microscope and the cell size was found to be bigger in the sludge medium than the TSB medium.

Type de document:
Mots-clés libres: CST; EPS production; fermentation; flocculation activity; serratia growth profile; wastewater sludge
Centre: Centre Eau Terre Environnement
Date de dépôt: 29 nov. 2016 21:18
Dernière modification: 22 déc. 2016 20:30
URI: http://espace.inrs.ca/id/eprint/3388

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