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In Vitro Inhibition of Hepatitis C Virus by Antisense Oligonucleotides in PBMC Compared to Hepatoma Cells

Youssef, Samar Samir; Fahmy, Ahmed Mohamed; Omran, Moataza Hassan; Mohamed, Amr Saad; El Desouki, Mohamed Ali; El-Awady, Mostafa K. (2014). In Vitro Inhibition of Hepatitis C Virus by Antisense Oligonucleotides in PBMC Compared to Hepatoma Cells BioMed Research International , vol. 2014 , nº 196712. p. 1-7. DOI: 10.1155/2014/196712.

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Résumé

AIM: To assess the efficiency of phosphorothioate antisense oligodeoxynucleotide 1 (S-ODN1) on HCV translation inhibition in PBMC compared to hepatoma cells in vitro for the first time.

MATERIALS AND METHODS: The study included 34 treatment naive HCV patients. IRES domain III and IV sequence variations were tested in 45 clones from 9 HCV patients. PBMC of HCV positive patients were subjected to S-ODN in vitro. Concomitantly HepG2 cells infected by the same patient's serum were also treated with S-ODN1 for 24 and 48 hours. Cellular RNA was tested for HCV plus and minus strands by reverse transcription polymerase chain reaction (RT-PCR).

RESULTS: Sequence variations were seen in HCV IRES domain III only while domain IV was conserved among all the tested patient's clones. S-ODN1 successfully inhibited HCV translation in HepG2 cells, while in PBMC inhibition was partial.

CONCLUSION: HCV IRES domain IV is more conserved than domain IIId in genotype 4 HCV patients. S-ODN against HCV IRES domain IV was not efficient to inhibit HCV translation in PBMC under the study conditions. Further studies testing other S-ODN targeting other HCV IRES domains in PBMC should be done.

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Centre: Centre INRS-Institut Armand Frappier
Date de dépôt: 26 juin 2017 19:43
Dernière modification: 26 juin 2017 19:43
URI: http://espace.inrs.ca/id/eprint/3135

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