Dépôt numérique

Bioavailability and Immunotoxicity of Silver Nanoparticles to the Freshwater Mussel Elliptio complanata

Gagné, François; Auclair, Julie; Fortier, Marlène; Bruneau, Audrey; Fournier, Michel; Turcotte, Patrice; Pilote, Martin; Gagnon, Christian (2013). Bioavailability and Immunotoxicity of Silver Nanoparticles to the Freshwater Mussel Elliptio complanata Journal of Toxicology and Environmental Health - Part A: Current Issues , vol. 76 , nº 13. p. 767-777. DOI: 10.1080/15287394.2013.818602.

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The purpose of this study was to examine the effects of Ag nanoparticles (nAg) of two different sizes (20 and 80 nm) and Ag+ on the immune system of the freshwater mussel Elliptio complanata. Mussels were exposed to increasing concentrations of nAg and dissolved Ag (AgNO3) for 48 h at 15°C and concentration of 0, 0.8, 4, or 20 μg/L. Immunocompetence was determined by hemocyte viability, phagocytosis, and cell cytotoxicity. Ag tissue loadings and levels of metallothioneins (MT), lipid peroxidation (LPO), and labile zinc (Zn) were also determined. Results revealed first that 20- and 80-nm nAg readily formed aggregates in freshwater. Ag was detected in soft tissues with each form of Ag with bioconcentration factors of 20, 9, and 7 for Ag+, 20-nm nAg, and 80-nm nAg, respectively. Significant induction in phagocytosis and decreased cell cytotoxicity were observed. All forms of Ag were able to induce LPO in gills and digestive glands at concentrations below those from the initial fraction of dissolved Ag. The effects of nAg on MT levels in mussels were not discernible from those of dissolved Ag, but the 80-nm was 25-fold more potent than 20-nm nAg in inducing MT. Multivariate analysis revealed that the global responses of the 20- and 80-nm nAg were generally similar to those of dissolved Ag. Data also demonstrated that nAg are bioavailable for mussels where the immune system is a target during early exposure to nanoparticles.

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Centre: Centre INRS-Institut Armand Frappier
Date de dépôt: 12 avr. 2017 15:52
Dernière modification: 12 avr. 2017 15:52
URI: http://espace.inrs.ca/id/eprint/2898

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