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Liquid chromatography/mass spectrometry analysis of mixtures of rhamnolipids produced by Pseudomonas aeruginosa strain 57RP grown on mannitol or naphthalene

Déziel, Éric; Lépine, François; Dennie, Danielle; Boismenu, Daniel L; Mamer, Orval A; Villemur, Richard (1999). Liquid chromatography/mass spectrometry analysis of mixtures of rhamnolipids produced by Pseudomonas aeruginosa strain 57RP grown on mannitol or naphthalene Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids , vol. 1440 , nº 2-3. p. 244-252. DOI: 10.1016/s1388-1981(99)00129-8.

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Résumé

Liquid chromatography/mass spectrometry using electrospray ionisation was used to analyse rhamnolipids produced by a Pseudomonas aeruginosa strain with mannitol or naphthalene as carbon source. Identification and quantification of 28 different rhamnolipid congeners was accomplished using a reverse-phase C18 column and a 30 min chromatographic run. Isomeric rhamnolipids that were not chromatographically resolved could be identified by interpretation of their mass spectra and their relative proportions estimated. The most abundant rhamnolipid produced on mannitol contained two rhamnoses and two 3-hydroxydecanoic acid groups. The most abundant rhamnolipid produced from naphthalene contained two rhamnoses and one 3-hydroxydecanoic acid group

Type de document:
Mots-clés libres: Liquid chromatography, Mass spectrometry, Pseudomonas aeruginosa, Rhamnolipid
Centre: Centre INRS-Institut Armand Frappier
Date de dépôt: 05 mai 2014 15:54
Dernière modification: 05 mai 2014 16:03
URI: http://espace.inrs.ca/id/eprint/2258

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