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Self-produced extracellular stimuli modulate the Pseudomonas aeruginosa swarming motility behaviour

Tremblay, Julien; Richardson, Anne-Pascale; Lépine, François et Déziel, Éric (2007). Self-produced extracellular stimuli modulate the Pseudomonas aeruginosa swarming motility behaviour Environmental Microbiology , vol. 9 , nº 10. pp. 2622-2630. DOI: 10.1111/j.1462-2920.2007.01396.x.

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Résumé

seudomonas aeruginosa presents three types of motilities: swimming, twitching and swarming. The latter is characterized by rapid and coordinated group movement over a semisolid surface resulting from morphological differentiation and intercellular interactions. A striking feature of P. aeruginosa swarming motility is the formation of migrating tendrils producing colonies with complex fractal-like patterns. Previous studies have shown that normal swarming motility is intimately related to the production of extracellular surface-active molecules: rhamnolipids (RLs), composed of monorhamnolipids (mono-RLs) and dirhamnolipids (di-RLs), and 3-(3-hydroxyalkanoyloxy) alkanoic acids (HAAs). Here, we report that (i) di-RLs attract active swarming cells while HAAs behave as strong repellents, (ii) di-RLs promote and HAAs inhibit tendril formation and migration, (iii) di-RLs and HAAs display different diffusion kinetics on a surface as di-RLs spread faster than HAAs in agar, (iv) di-RLs and HAAs have no effect on swimming cells, suggesting that swarming cells are different from swimming cells not only in morphology but also at the regulatory level and (v) mono-RLs act as wetting agents. We propose a model explaining how HAAs and di-RLs together modulate the behaviour of swarming migrating cells by acting as self-produced negative and positive chemotactic-like stimuli

Type de document: Article
Mots-clés libres: RHAMNOLIPID BIOSURFACTANT SYNTHESIS, 3-(3-HYDROXYALKANOYLOXY)ALKANOIC ACIDS HAAS, BACILLUS-SUBTILIS, TWITCHING MOTILITIES, ESCHERICHIA-COLI, GENE-EXPRESSION, STRAIN 57RP, BIOSYNTHESIS; PATTERNS, ARCHITECTURE
Centre: Centre INRS-Institut Armand Frappier
Date de dépôt: 01 mai 2014 16:10
Dernière modification: 01 mai 2014 16:10
URI: https://espace.inrs.ca/id/eprint/2227

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