Dépôt numérique

Recherches faites avec les mycobactéries marquées. VI. Dispersion du BCG marque (au 32P) chez le cobaye

Sternberg, Joseph et Frappier, Armand (1956). Recherches faites avec les mycobactéries marquées. VI. Dispersion du BCG marque (au 32P) chez le cobaye Annales de l'Institut Pasteur , vol. 90 , nº 5. pp. 533-574.

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The study of microbial dispersion with the aid of labelled microbes must be done by superimpozing a biological control (microbial numeration) upon the resul!s offered by the radioactivity assay. The ratio 32Pfmicrobe has been named mict·ohial specifie activity.

In normal guine.a pig injected with 108 microbes, .almost half of the injected dosis has been found at the inoculation site, whereas the microbial mass recuperated in all the other tissues together averages at most 1% of the total injected. The number of living microbes found at the inoculation site of the vaccinated animal does not differ significantly from that of the control, while in the other examined tissues (liver, spleen, lungs) the number of microbes is considerably diminished in vaccin.ated (100 to 500 times) as compared to the controls.

The radioactivity assay of the tissues gave different results than that of the direct microbial count, namely a considerable increase of the tissular concentration of the isotope, which averages nearly 50%. of the injected dosis, instead of the 1% of the tissular microbes as found by the bacteriological procedure.

The vaccination with BCG does not seem lo aller the pattem of distribution of the isotope in the tissues, but the microbial specifie activity is eonsiderably incre.ased in vaccinated animals, because of the diminution of· the living microbial units in the examined tissues.

In animals înjected with heat-killed labelled BCG, the pattern of distribution of 32P d1ffers from that in animais injected with living microbes, especially in the skin, which concentrates more 32P m the former case. This is even more marked in vaccinated animais.

The pattern of tissular distribution of 32P in experiments with labelled microbes is different from the distribution schema of the mineral radiophosphate. In animais injected simultaneously With 32PO4.,Na2H and living non labelled BCG, the highest percentage of the isotope (38%) was excreted in the urine, and almost an equal part was fixed in the bones ; the concentration at the inoculation site is far less important than in the former experiments.

There is an acceleration of the phosphate turnover in vaccinaled animais, noted in experiments with labelled microbes as weil as in the experiments with mineral radiophosphate.

Type de document: Article
Informations complémentaires: Recueil de tiré-à-part de la bibliothèque: A0117
Mots-clés libres: BCG VACCINATION/experimental; PHOSPHORUS/radioactive; BCG vaccine; phosphate intake; phosphorus; guinea pig; Mycobacterium; Mycobacterium bovis; vaccination; Guinea Pigs; Phosphorus Radioisotopes; Phosphorus, Dietary
Centre: Centre INRS-Institut Armand Frappier
Date de dépôt: 10 févr. 2020 16:09
Dernière modification: 10 févr. 2020 16:09
URI: https://espace.inrs.ca/id/eprint/8398

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