Dépôt numérique

Aspergillus-Pseudomonas interaction, relevant to competition in airways.

Sass, Gabriele, Nazik, Hasan, Penner, John, Ansari, Shajia Rahman, Clemons, Karl V, Groleau, Marie-Christine, Dietl, Anna-Maria, Visca, Paolo, Haas, Hubertus, Déziel, Éric ORCID: https://orcid.org/0000-0002-4609-0115 et Stevens, David A (2019). Aspergillus-Pseudomonas interaction, relevant to competition in airways. Medical Mycology , vol. 57 , nº Suppl. 2. S228-S232. DOI: 10.1093/mmy/myy087.

Ce document n'est pas hébergé sur EspaceINRS.


In airways of immunocompromised patients and individuals with cystic fibrosis, Pseudomonas aeruginosa and Aspergillus fumigatus are the most common opportunistic bacterial and fungal pathogens. Both pathogens form biofilms and cause acute and chronic illnesses. Previous studies revealed that P. aeruginosa is able to inhibit A. fumigatus biofilms in vitro. While numerous P. aeruginosa molecules have been shown to affect A. fumigatus, there never has been a systematic approach to define the principal causative agent. We studied 24 P. aeruginosa mutants, with deletions in genes important for virulence, iron acquisition, or quorum sensing, for their ability to interfere with A. fumigatus biofilms. Cells, planktonic or biofilm culture filtrates of four P. aeruginosa mutants, pvdD-pchE-, pvdD-, lasR-rhlR-, and lasR-, inhibited A. fumigatus biofilm metabolism or planktonic A. fumigatus growth significantly less than P. aeruginosa wild type. The common defect of these four mutants was a lack in the production of the P. aeruginosa siderophore pyoverdine. Pure pyoverdine affected A. fumigatus biofilm metabolism, and restored inhibition by the above mutants. In lungs from cystic fibrosis patients, pyoverdine production and antifungal activity correlated. The key inhibitory mechanism for pyoverdine was iron-chelation and denial of iron to A. fumigatus. Further experiments revealed a counteracting, self-protective mechanism by A. fumigatus, based on A. fumigatus siderophore production.

Type de document: Article
Mots-clés libres: Aspergillus fumigatus ; Pseudomonas aeruginosa ; cystic fibrosis; microbial interaction; pyoverdine
Centre: Centre INRS-Institut Armand Frappier
Date de dépôt: 11 janv. 2020 15:29
Dernière modification: 07 févr. 2022 15:56
URI: https://espace.inrs.ca/id/eprint/8172

Actions (Identification requise)

Modifier la notice Modifier la notice