Bench-scale production of enzymes from the hydrocarbonoclastic bacteria Alcanivorax borkumensis and biodegradation tests.

Kadri, Tayssir; Magdouli, Sara; Rouissi, Tarek; Brar, Satinder Kaur; Daghrir, Rimeh et Lauzon, Jean-Marc (2018). Bench-scale production of enzymes from the hydrocarbonoclastic bacteria Alcanivorax borkumensis and biodegradation tests. Journal of Biotechnology , vol. 283 . pp. 105-114. DOI: 10.1016/j.jbiotec.2018.07.039.

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Résumé

This study investigates motor oil (3, 5, 7.5 and 10% (v v− ⁻¹)) as a sole carbon source for the production of Alcanivorax borkumensis in shake flasks and a 5 L bench-scale fermenter in comparison to the standard media. Shake flask studies showed a significant and higher cell growth (p=0.000038), lipase (p = 0.006900) and alkane hydroxylase production (p = 0.000921) by Alcanivorax borkumensis when motor oil was used as the substrate. Based on Tukey post-hoc tests, 5% motor oil concentration was selected as the optimal substrate concentration. The 5 L fermenter experiments conducted using motor oil at 5% (v v⁻¹) concentration, under controlled conditions exhibited significant and higher alkane hydroxylase and lipase activities (55.6 U mL⁻¹ (p = 0.018418) and 208.30 U mL⁻¹ (p =  0.020087), respectively) as compared with those of motor oil at 3% (v v⁻¹) and n-hexadecane at 3% (v v⁻¹) concentration which was used as control. Cell growth was significantly higher when motor oil (3 or 5%) was used as a substrate (p =  0.024705). Enzymatic degradation tested on two different polycyclic aromatic hydrocarbons (PAHs) contaminated groundwaters showed 37.4% removal after 5 days with a degradation rate of 196.6 ppb day⁻¹ and 82.8% removal after 10 days with a degradation rate of 217.54 ppb day⁻¹ for the 1st site and an almost complete biodegradation with 95% removal and 499.02 ppb day−1 removal rate after only 5 days for the 2nd site.

Type de document:	Article
Mots-clés libres:	fermentation; Alcanivorax borkumensis; alkane hydroxylase; lipase; PAHs
Centre:	Centre Eau Terre Environnement
Date de dépôt:	18 oct. 2018 18:47
Dernière modification:	30 juill. 2019 04:00
URI:	https://espace.inrs.ca/id/eprint/7661

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