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Comparison of peripheral oxidative markers between Mild Cognitive Impairement (MCI) and Alzheimer’s patients

Perrotte, Morgane, Le Page, Aurélie, Camponova, Pamela, Fulop, Tamas, Rassart, Éric et Ramassamy, Charles . Comparison of peripheral oxidative markers between Mild Cognitive Impairement (MCI) and Alzheimer’s patients In: 13ème colloque annuelle du centre de recherche Biomed, 28 avril 2017, Cœur des Sciences, UQAM, Montréal (Québec).

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Background: Alzheimer's disease (AD) is a progressive neurodegenera- tive diseasecharacterized by memory loss and multiple cognitiveimpairments. Current- ly, there are no drugs or agents that can delay and/or prevent the progression of AD. Moreover, there are no peripheral biomarkers that can detect AD early in its pathogenesis. The identification of biomarker is crucial to identify individuals with high risk to develop AD. Oxidative damages occurred early in the brain from AD and they were found to be elevated in the brain, cerebrospinal fluid and plasma. However, a specific pattern of peripheral oxidative markers in mild cognitive impairment (MCI) and in AD remains to be determined. This study aimed to establish a comparison of redox bi- omarkers in plasma between MCI and AD patients at different stages of the disease.

Methods: In plasma from patients with MCI and AD at each disease stage and from age - matched controls, we have measured the antioxidant capacity (AC) by electro- chemical detection (Free Radical analyzer Apollo 4000), quantified the total reduced protein thiols with the Ellman’s reagent (5,5' - dithiobis - [2 - nitrobenzoic acid], DTNB), evaluated Klotho levels using a sandwich enzyme - linked immunosorbent assay kit (R'D Systems, Inc.) and analyzed Apolipoprotein D (ApoD), Apolipoprotein J (ApoJ) and protein carbonyls by Western Blot.

Results: The AC is significantly decreased in MCI and AD groups compared to con- trol group. Protein carbonyls levels were similar in controls and MCI patients but were increased progressively increased from mild AD patients. Klotho and ApoJ levels increased in MCI as well as in mild and moderate AD. Interestingly, the AC levels are correlated with the cognitive scores of MoCa and MMSE test. Additionally, the protein carbonyls levels are correlated with the MoCA scores only. Moreover, there is a correlation between ApoJ levels and cognitive scores.

Conclusion: Our results indicate that the antioxidant capacity decreases from MCI patients while Klotho and ApoJ levels increase. The protein carbonyls levels are raised later in AD groups. Interestingly, these peripheral oxidative markers are correlated with clinical score. These results show that peripheral specific oxidative pattern has a potential as blood - based biomarkers for MCI and progression of AD.

Type de document: Document issu d'une conférence ou d'un atelier
Informations complémentaires: Présentation par affiche
Mots-clés libres: -
Centre: Centre INRS-Institut Armand Frappier
Date de dépôt: 26 déc. 2017 18:13
Dernière modification: 07 déc. 2020 14:16
URI: https://espace.inrs.ca/id/eprint/6637

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