Dépôt numérique

Role of activation-induced cytidine deaminase (AID) in the context of L. donovaniinfection

Silva-Barrios, Sasha et Stäger, Simona . Role of activation-induced cytidine deaminase (AID) in the context of L. donovaniinfection In: 16e symposium annuel de parasitologie moléculaire du Québec, 6-7 juin 2016, Université McGill, Montréal, QC.

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The protozoan parasite Leishmania donovani, one of the causative agents of visceral leishmaniasis (VL), is known to induce polyclonal B cell activation and hypergammaglobulinemia. B cell activation by the parasite is considered to have a negative effect on the infection; mice deficient in B cells are highly resistant to VL. Also, it has been reported that IgM and IgG exacerbates VL. Recently we have shown that innate B cell activation during chronic VL promotes hypergammaglobulinemia and exacerbates disease through a positive feedback loop involving Type I IFN. High levels of low avidity IgG have been described during the course of the infection. Somatic hypermutuation (SHM) is one of the principal events that allow the development of high affinity antibodies, one of the enzymes involved in this process is the activation-induced cytidine deaminase (AID). Here we evaluate the role of AID during experimental VL. AID-deficient mice were infected with L. dononovani amastigotes and different parameters during the course of the infection were evaluated. Preliminary data suggest that upregulation of co-stimulatory molecules in B cell and L. donovani-specific Th1 responses do not seem to be affected by the lack of AID during the infection. Nevertheless, we found that specific antibody titres against L. donovani as well as parasite burden were lower in AID deficient mice compared to WT mice. Taken together, these results suggest that activation of AID is important in VL exacerbation.

Type de document: Document issu d'une conférence ou d'un atelier
Informations complémentaires: Affiche scientifique
Mots-clés libres: -
Centre: Centre INRS-Institut Armand Frappier
Date de dépôt: 01 mai 2018 19:43
Dernière modification: 01 mai 2018 19:56
URI: https://espace.inrs.ca/id/eprint/5926

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