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Ultrastructural Characterization of Zika Virus Replication Factories


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Cortese, Mirko, Goellner, Sarah, Acosta, Eliana Gisela, Neufeldt, Christopher John, Oleksiuk, Olga, Lampe, Marko, Haselmann, Uta, Funaya, Charlotta, Schieber, Nicole, Ronchi, Paolo, Schorb, Martin, Pruunsild, Priit, Schwab, Yannick, Chatel-Chaix, Laurent ORCID: https://orcid.org/0000-0002-7390-8250, Ruggieri, Alessia et Bartenschlager, Ralf (2017). Ultrastructural Characterization of Zika Virus Replication Factories Cell Reports , vol. 18 , nº 9. p. 2113-2123. DOI: 10.1016/j.celrep.2017.02.014.

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A global concern has emerged with the pandemic spread of Zika virus (ZIKV) infections that can cause severe neurological symptoms in adults and newborns. ZIKV is a positive-strand RNA virus replicating in virus-induced membranous replication factories (RFs). Here we used various imaging techniques to investigate the ultrastructural details of ZIKV RFs and their relationship with host cell organelles. Analyses of human hepatic cells and neural progenitor cells infected with ZIKV revealed endoplasmic reticulum (ER) membrane invaginations containing pore-like openings toward the cytosol, reminiscent to RFs in Dengue virus-infected cells. Both the MR766 African strain and the H/PF/2013 Asian strain, the latter linked to neurological diseases, induce RFs of similar architecture. Importantly, ZIKV infection causes a drastic reorganization of microtubules and intermediate filaments forming cage-like structures surrounding the viral RF. Consistently, ZIKV replication is suppressed by cytoskeleton-targeting drugs. Thus, ZIKV RFs are tightly linked to rearrangements of the host cell cytoskeleton.

Type de document: Article
Mots-clés libres: Zika virus; electron microscopy; electron tomography; flavivirus; human neural progenitor cells; intermediate filaments; live-cell imaging; microtubules; replication factories; replication organelles
Centre: Centre INRS-Institut Armand Frappier
Date de dépôt: 20 févr. 2019 20:42
Dernière modification: 21 févr. 2022 17:17
URI: https://espace.inrs.ca/id/eprint/5338

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