Dépôt numérique

Dietary sodium protects fish against copper-induced olfactory impairment.

Azizishirazi, Ali, Dew, William A., Bougas, Bérénice, Bernatchez, Louis et Pyle, Greg G. (2015). Dietary sodium protects fish against copper-induced olfactory impairment. Aquatic Toxicology , vol. 161 . p. 1-9. DOI: 10.1016/j.aquatox.2015.01.017.

Ce document n'est pas hébergé sur EspaceINRS.


Exposure to low concentrations of copper impairs olfaction in fish. To determine the transcriptional changes in the olfactory epithelium induced by copper exposure, wild yellow perch (Perca flavescens) were exposed to 20 μg/L of copper for 3 and 24 h. A novel yellow perch microarray with 1000 candidate genes was used to measure differential gene transcription in the olfactory epithelium. While three hours of exposure to copper changed the transcription of only one gene, the transcriptions of 70 genes were changed after 24 h of exposure to copper. Real-time PCR was utilized to determine the effect of exposure duration on two specific genes of interest, two sub-units of Na/K-ATPase. At 24 and 48 h, Na/K-ATPase transcription was down-regulated by copper at olfactory rosettes. As copper-induced impairment of Na/K-ATPase activity in gills can be ameliorated by increased dietary sodium, rainbow trout (Oncorhynchus mykiss) were used to determine if elevated dietary sodium was also protective against copper-induced olfactory impairment. Measurement of the olfactory response of rainbow trout using electro-olfactography demonstrated that sodium was protective of copper-induced olfactory dysfunction. This work demonstrates that the transcriptions of both subunits of Na/K-ATPase in the olfactory epithelium of fish are affected by Cu exposure, and that dietary Na protects against Cu-induced olfactory dysfunction.

Type de document: Article
Mots-clés libres: olfaction; olfactory toxicity; copper; microarray; real-time PCR; electro-olfactogrphy
Centre: Centre Eau Terre Environnement
Date de dépôt: 25 avr. 2018 15:58
Dernière modification: 25 avr. 2018 15:58
URI: https://espace.inrs.ca/id/eprint/4293

Actions (Identification requise)

Modifier la notice Modifier la notice