Dépôt numérique
RECHERCHER

Metal (Ag, Cd, Cu, Ni, Tl, and Zn) Binding to Cytosolic Biomolecules in Field-Collected Larvae of the Insect Chaoborus.

Rosabal, Maikel, Mounicou, Sandra, Hare, Landis et Campbell, Peter G. C. (2016). Metal (Ag, Cd, Cu, Ni, Tl, and Zn) Binding to Cytosolic Biomolecules in Field-Collected Larvae of the Insect Chaoborus. Environmental Science & Technology , vol. 50 , nº 6. p. 3247-3255. DOI: 10.1021/acs.est.5b05961.

Ce document n'est pas hébergé sur EspaceINRS.

Résumé

We characterized the biomolecules involved in handling cytosolic metals in larvae of the phantom midge (Chaoborus) collected from five mining-impacted lakes by determining the distribution of Ag, Cd, Cu, Ni, Tl, and Zn among pools of various molecular weights (HMW: high molecular weight, >670?40 kDa; MMW: medium molecular weight, 40? <1.3 kDa; LMW: low molecular weight, <1.3 kDa). Appreciable concentrations of nonessential metals were found in the potentially metal-sensitive HMW (Ag and Ni) and LMW (Tl) pools, whereas the MMW pool, which includes metallothioneins (MTs) and metallothionein-like proteins and peptides (MTLPs), appears to be involved in Ag and Cd detoxification. Higher-resolution fractionation of the heat-stable protein (HSP) fraction revealed further differences in the partitioning of nonessential metals (i.e., Ag = Cd ? Ni ? Tl). These results provide unprecedented details about the metal-handling strategies employed by a metal-tolerant, freshwater animal in a field situation.

Type de document: Article
Mots-clés libres: Metals; biomolecules; freshwater larvae; chaoborus; biomonitoring;
Centre: Centre Eau Terre Environnement
Date de dépôt: 20 déc. 2016 16:42
Dernière modification: 12 nov. 2018 16:44
URI: https://espace.inrs.ca/id/eprint/4139

Actions (Identification requise)

Modifier la notice Modifier la notice