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Evidence of metabolic shift on hydrogen, ethanol and 1,3-propanediol production from crude glycerol by nitrogen sparging under micro-aerobic conditions using co-culture of Enterobacter aerogenes and Clostridium butyricum.

Pachapur, Vinayak Laxman, Sarma, Saurabh Jyoti, Brar, Satinder Kaur, Le Bihan, Yann, Buelna, Gerardo et Soccol, Carlos Ricardo (2015). Evidence of metabolic shift on hydrogen, ethanol and 1,3-propanediol production from crude glycerol by nitrogen sparging under micro-aerobic conditions using co-culture of Enterobacter aerogenes and Clostridium butyricum. Science of The Total Environment , vol. 40 , nº 28. p. 8669-8676. DOI: 10.1016/j.ijhydene.2015.05.024.

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Résumé

Hydrogen (H₂), a possible future clean energy carrier, requires process-based improvement routes for cutting down the production cost. The impact of nitrogen (N₂) sparging on H₂ production during co-culture system of Enterobacter aerogenes and Clostridium butyricum from crude glycerol (CG) was studied to reduce the overall process cost. H₂ production using 1% CG under nitrogen sparged medium before autoclaving resulted in 1.2 mmol-H₂/mol of glycerol in comparison to 1.5 mmol-H₂/mol of glycerol without sparging. In the presence of air ranging from 5 mL to 75 mL in the headspace volume, H₂ production increased to a maximum of 26.14 mmol/L with 1.4 g/L of ethanol production. The concentration of 1,3-propanediol with N2 sparging was around 3.0 g/L and decreased to 0.5 g/L due to presence of 75 mL of air in the headspace. This observation can be attributed to a shift from reductive to oxidative metabolism of glycerol. A process-based improvement strategy to optimize H₂ formation resulted in metabolic pathway shift from reductive to oxidative with increase in H₂ production. Synergistic influence of co-culture system in absence of expensive reducing agent and without nitrogen sparging step can offer a better process-based economic strategy for H₂ production, minimize the metabolite production and increase field-scale application of biodiesel plant.

Type de document: Article
Mots-clés libres: co-culture; crude glycerol; ethanol; hydrogen; nitrogen sparging; 1,3-Propanediol
Centre: Centre Eau Terre Environnement
Date de dépôt: 23 avr. 2018 17:46
Dernière modification: 23 avr. 2018 17:46
URI: https://espace.inrs.ca/id/eprint/3900

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