Yadav, Jay Shankar Singh, Bezawada, Jyothi, Yan, Song, Tyagi, Rajeshwar Dayal et Surampalli, Rao Y. (2014). Permeabilization of Kluyveromyces marxianus with mild detergent for whey lactose hydrolysis and augmentation of mixed culture. Applied Biochemistry and Biotechnology , vol. 172 , nº 6. p. 3207-3222. DOI: 10.1007/s12010-014-0755-z.
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Cheese whey is a by-product of cheese-manufacturing industries, and the utilization of whey is a challenging problem either to use it or dispose it, because only few microorganisms can metabolize the whey lactose. Enzymatic hydrolysis of whey lactose to glucose and galactose by β-galactosidase is the approach for biotechnological application. Kluyveromyces marxianus cells were permeabilized with non-toxic, biodegradable, anionic detergent N-lauroyl sarcosine (N-LS) for the enzyme activity. The permeabilization process parameters (N-LS concentration, solvent volume, temperature and incubation time) were optimized. The maximum β-galactosidase activity of 1,220 IU/g dry weight was obtained using permeabilized cells under optimized conditions. Moreover, viability of the permeabilized cells was also evaluated, which showed that cells were alive; however, viability was reduced by two log cycles. The permeabilized cells were evaluated for whey lactose hydrolysis. The maximum lactose hydrolysis of 91 % was observed with 600 mg (dry cell weight/100 mL) in whey powder (5 % w/v) solution at 180-min incubation, pH 6.5 and 30°C. Further, the hydrolyzed whey was evaluated for amelioration of growth of non-lactose-consuming yeast Saccharomyces cerevisiae. S. cerevisiae was able to grow in hydrolyzed whey simultaneously with K. marxianus. The study confirmed that N-LS could be used to permeabilize K. marxianus cells to make available the enzyme activity.
| Type de document: | Article |
|---|---|
| Mots-clés libres: | kluyveromyces marxianus; lactose hydrolysis; mixed culture; N-lauroyl sarcosine; permeabilization; single-cell protein; β-galactosidase |
| Centre: | Centre Eau Terre Environnement |
| Date de dépôt: | 11 avr. 2018 18:30 |
| Dernière modification: | 11 avr. 2018 18:30 |
| URI: | https://espace.inrs.ca/id/eprint/3695 |
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