Sánchez-Marin, Paula; Fortin, Claude et Campbell, Peter G. C. (2014). Lead (Pb) and copper (Cu) share a common uptake transporter in the unicellular alga Chlamydomonas reinhardtii. BioMetals , vol. 27 , nº 1. pp. 173-181. DOI: 10.1007/s10534-013-9699-y.
Ce document n'est pas hébergé sur EspaceINRS.Résumé
The unicellular alga Chlamydomonas reinhardtii has a very high rate of lead (Pb) internalization and is known to be highly sensitive to dissolved Pb. However, the transport pathway that this metal uses to cross cellular membranes in microalgae is still unknown. To identify the Pb²⁺ transport pathway in C. reinhartdii, we performed several competition experiments with environmentally relevant concentrations of Pb²⁺ (~10 nM) and a variety of divalent cations. Among the essential trace metals tested, cobalt, manganese, nickel and zinc had no effect on Pb internalization. A greater than tenfold increase in the concentrations of the major ions calcium and magnesium led to a slight decrease (~34 %) in short-term Pb internalization by the algae. Copper (Cu) was even more effective: at a Cu concentration 50 times higher than that of Pb, Pb internalization by the algae decreased by 87 %. Pre-exposure of the algae to Cu showed that the effect was not due to a physiological effect of Cu on the algae, but rather to competition for the same transporter. A reciprocal effect of Pb on Cu internalization was also observed. These results suggest that Cu and Pb share a common transport pathway in C. reinhardtii at environmentally relevant metal concentrations.
| Type de document: | Article |
|---|---|
| Mots-clés libres: | competition; microalgae; trace metals; transmembrane transport |
| Centre: | Centre Eau Terre Environnement |
| Date de dépôt: | 11 avr. 2018 15:57 |
| Dernière modification: | 16 avr. 2018 13:41 |
| URI: | https://espace.inrs.ca/id/eprint/3677 |
Gestion Actions (Identification requise)
 |
Modifier la notice |