Secondary conformational conversion is involved in glycosaminoglycans-mediated cellular uptake of the cationic cell-penetrating peptide PACAP

Tchoumi Neree, Armelle; Nguyen, Phuong Trang; Chatenet, David; Fournier, Alain et Bourgault, Steve (2014). Secondary conformational conversion is involved in glycosaminoglycans-mediated cellular uptake of the cationic cell-penetrating peptide PACAP FEBS Letters , vol. 588 , nº 24. pp. 4590-4596. DOI: 10.1016/j.febslet.2014.10.029.

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Résumé

Glycosaminoglycans (GAGs) contribute to the cellular uptake of cationic cell-penetrating peptides (CPPs). However, molecular details about the contributions of GAGs in CPP internalization remain unclear. In this study, we examined the cellular uptake mechanism of the arginine-rich CPP pituitary adenylate-cyclase-activating polypeptide (PACAP). We observed that the uptake efficacy of PACAP is dependent on the expression of cell surface GAGs. As the binding of PACAP to sulfated GAGs induced a random coil-to-α-helix conformational conversion, we investigated the role of the helical formation in PACAP internalization. Whereas this secondary structure was not crucial for efficient internalization in GAGs-deficient cells, PACAP α-helix was essential for GAGs-dependent uptake.

Type de document:	Article
Mots-clés libres:	Cell-penetrating peptide; Cellular uptake; Glycosaminoglycan; Pituitary adenylate cyclase-activating polypeptide α-Helix
Centre:	Centre INRS-Institut Armand Frappier
Date de dépôt:	23 juin 2017 04:20
Dernière modification:	23 juin 2017 04:21
URI:	https://espace.inrs.ca/id/eprint/3123

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