Genipin cross-linked nanocomposite films for the immobilization of antimicrobial agent

Khan, Avik, Salmieri, Stéphane, Fraschini, Carole, Bouchard, Jean, Riedl, Bernard et Lacroix, Monique (2014). Genipin cross-linked nanocomposite films for the immobilization of antimicrobial agent ACS Applied Materials & Interfaces , vol. 6 , nº 17. p. 15232-15242. DOI: 10.1021/am503564m.

Prévisualisation

PDF (Le document est disponible dans la thèse en version article accepté à partir de la page 85 à l’adresse URL suivante : http://espace.inrs.ca/id/eprint/2746) - Image de couverture Télécharger (226kB) | Prévisualisation

Résumé

Cellulose nanocrystal (CNC) reinforced chitosan based antimicrobial films were prepared by immobilizing nisin on the surface of the films. Nanocomposite films containing 18.65 mug/cm(2) of nisin reduced the count of L. monocytogenes by 6.73 log CFU/g, compared to the control meat samples (8.54 log CFU/g) during storage at 4 degrees C in a Ready-To-Eat (RTE) meat system. Film formulations containing 9.33 mug/cm(2) of nisin increased the lag phase of L. monocytogenes on meat by more than 21 days, whereas formulations with 18.65 mug/cm(2) completely inhibited the growth of L. monocytogenes during storage. Genipin was used to cross-link and protect the activity of nisin during storage. Nanocomposite films cross-linked with 0.05% w/v genipin exhibited the highest bioactivity (10.89 mug/cm(2)) during the storage experiment, as compared to that of the un-cross-linked films (7.23 mug/cm(2)). Genipin cross-linked films were able to reduce the growth rate of L. monocytogenes on ham samples by 21% as compared to the un-cross-linked films. Spectroscopic analysis confirmed the formation of genipin-nisin-chitosan heterocyclic cross-linked network. Genipin cross-linked films also improved the swelling, water solubility, and mechanical properties of the nanocomposite films.

Type de document:	Article
Mots-clés libres:	Listeria monocytogenes; antimicrobial films; chitosan; genipin; nisin; surface modification
Centre:	Centre INRS-Institut Armand Frappier
Date de dépôt:	01 juin 2017 15:35
Dernière modification:	01 juin 2017 15:35
URI:	https://espace.inrs.ca/id/eprint/3067

Actions (Identification requise)

Modifier la notice