Case report: the use of annexin V coupled with magnetic activated cell sorting in cryopreserved spermatozoa from a male cancer survivor: healthy twin newborns after two previous ICSI failures

Herrero, Maria Belen; Delbès, Géraldine; Chung, Jin-Tae; Son, Weon-Young; Holzer, Hananel; Buckett, William et Chan, Peter T. K. (2013). Case report: the use of annexin V coupled with magnetic activated cell sorting in cryopreserved spermatozoa from a male cancer survivor: healthy twin newborns after two previous ICSI failures Journal of Assisted Reproduction and Genetics , vol. 30 , nº 11. pp. 1415-9. DOI: 10.1007/s10815-013-0086-y.

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Résumé

The symbols and special characters used in the original abstract could not be transcribed due to technical problems. Please use the PDF version to read the abstract. PURPOSE: The aim of the study is to report successful outcome (live births) after sperm sorting with annexin V-MACS on cryopreserved spermatozoa with high level of sperm DNA fragmentation from a cancer patient survivor. METHODS: Cryopreserved spermatozoa were sorted with annexin V-MACS prior to ICSI. Sperm DNA fragmentation was evaluated by SCSA((R)) and TUNEL. RESULTS: The couple had two previous IVF/ICSI cycles failures using sperm cryopreserved before cancer treatment. On third ICSI cycle attempt results were as follow: pre-annexin V-MACS sperm quality: 10 x 10(6)/ml, 3.3 % progressive motility, 1 % normal forms, TUNEL: 72.5 % positive cells, SCSA((R)): 76.6 % DFI. Post-annexin V-MACS sperm quality: 2.8 x 10(6)/ml, 10 % progressive motility, TUNEL: 58.8 % positive cells. Eight metaphase II oocytes were collected, 4 fertilized, 2 embryos were transferred on day 3 and healthy twins were born (1 boy, 1 girl). CONCLUSIONS: Annexin V-MACS technique could be a potential tool to improve sperm quality on cryopreserved spermatozoa of cancer patient and improve ICSI outcome.

Type de document:	Article
Mots-clés libres:	Cancer survivor; Cryopreserved sperm; Annexin V-MACS
Centre:	Centre INRS-Institut Armand Frappier
Date de dépôt:	16 mai 2017 19:39
Dernière modification:	08 juin 2023 17:47
URI:	https://espace.inrs.ca/id/eprint/2917

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