Cabrera-Valladares, Natividad; Richardson, Anne-Pascale; Olvera, Clarita; Trevino, Luis Gerardo; Lépine, François; Déziel, Éric et Soberón-Chávez, Gloria (2006). Monorhamnolipids and 3-(3-hydroxyalkanoyloxy) alkanoic acids (HAAs) production using Escherichia coli as a heterologous host Applied Microbiology and Biotechnology , vol. 73 , nº 1. pp. 187-194. DOI: 10.1007/s00253-006-0468-5.
Ce document n'est pas hébergé sur EspaceINRS.Résumé
Pseudomonas aeruginosa produces the biosurfactants rhamnolipids and 3-(3-hydroxyalkanoyloxy)alkanoic acids (HAAs). In this study, we report the production of one family of rhamnolipids, specifically the monorhamnolipids, and of HAAs in a recombinant Escherichia coli strain expressing P. aeruginosa rhlAB operon. We found that the availability in E. coli of dTDP-l-rhamnose, a substrate of RhlB, restricts the production of monorhamnolipids in E. coli. We present evidence showing that HAAs and the fatty acid dimer moiety of rhamnolipids are the product of RhlA enzymatic activity. Furthermore, we found that in the recombinant E. coli, these compounds have the same chain length of the fatty acid dimer moiety as those produced by P. aeruginosa. These data suggest that it is RhlAB specificity, and not the hydroxyfatty acid relative abundance in the bacterium, that determines the profile of the fatty acid moiety of rhamnolipids and HAAs. The rhamnolipids level produced in recombinant E. coli expressing rhlAB is lower than the P. aeruginosa level and much higher than those reported by others in E. coli, showing that this metabolic engineering strategy lead to an increased rhamnolipids production in this heterologous host
| Type de document: | Article |
|---|---|
| Mots-clés libres: | PSEUDOMONAS AERUGINOSA, ENZYMATIC SYNTHESIS, L-RHAMNOSE, BIOSYNTHESIS, RHAMNOLIPIDS, QUORUM, GENE, BIOSURFACTANT, CLONING, PRECURSORS |
| Centre: | Centre INRS-Institut Armand Frappier |
| Date de dépôt: | 01 mai 2014 18:49 |
| Dernière modification: | 01 mai 2014 18:49 |
| URI: | https://espace.inrs.ca/id/eprint/2238 |
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