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Convergent evolution of hyperswarming leads to impaired biofilm formation in pathogenic bacteria


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van Ditmarsch, Dave; Boyle, Kerry E.; Sakhtah, Hassan; Oyler, Jennifer E.; Nadell, Carey D.; Déziel, Éric; Dietrich, Lars E.P. et Xavier, Joao B. (2013). Convergent evolution of hyperswarming leads to impaired biofilm formation in pathogenic bacteria Cell Reports , vol. 4 , nº 4. pp. 697-708. DOI: 10.1016/j.celrep.2013.07.026.

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Most bacteria in nature live in surface-associated communities rather than planktonic populations. Nonetheless, how surface-associated environments shape bacterial evolutionary adaptation remains poorly understood. Here, we show that subjecting Pseudomonas aeruginosa to repeated rounds of swarming, a collective form of surface migration, drives remarkable parallel evolution toward a hyperswarmer phenotype. In all independently evolved hyperswarmers, the reproducible hyperswarming phenotype is caused by parallel point mutations in a flagellar synthesis regulator, FleN, which locks the naturally monoflagellated bacteria in a multiflagellated state and confers a growth rate-independent advantage in swarming. Although hyperswarmers outcompete the ancestral strain in swarming competitions, they are strongly outcompeted in biofilm formation, which is an essential trait for P. aeruginosa in environmental and clinical settings. The finding that evolution in swarming colonies reliably produces evolution of poor biofilm formers supports the existence of an evolutionary trade-off between motility and biofilm formation

Type de document: Article
Mots-clés libres: amino acid sequence; article; bacterial genome; bacterial phenomena and functions; bacterial strain; biofilm; controlled study; gene sequence; growth rate; hyperswarming; nonhuman; phenotype; point mutation; priority journal; Pseudomonas aeruginosa; single nucleotide polymorphism; transmission electron microscopy
Centre: Centre INRS-Institut Armand Frappier
Date de dépôt: 29 avr. 2014 20:43
Dernière modification: 29 avr. 2014 20:43
URI: https://espace.inrs.ca/id/eprint/2186

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