Nicastro, Gianlucca G.; Kaihami, Gilberto Hideo; Pereira, Thays O; Meireles, Diogo A.; Groleau, Marie-Christine; Déziel, Éric et Baldini, Regina L. (2014). Cyclic-di-GMP levels affect Pseudomonas aeruginosa fitness in the presence of imipenem Environmental Microbiology , vol. 16 , nº 5. pp. 1321-1333. DOI: 10.1111/1462-2920.12422.
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A large number of genes coding for enzymes predicted to synthesize and degrade 3′–5′-cyclic diguanylic acid (c-di-GMP) is found in most bacterial genomes and this dinucleotide emerged as an intracellular signal-controlling bacterial behaviour. An association between high levels of c-di-GMP and antibiotic resistance may be expected because c-di-GMP regulates biofilm formation and this mode of growth leads to enhanced antibiotic resistance. However, a clear understanding of this correlation has not been established. We found that increased levels of c-di-GMP in Pseudomonas aeruginosa improve fitness in the presence of imipenem, even when grown as planktonic cells. P. aeruginosa post-transcriptionally regulates the amounts of five porins in response to c-di-GMP, including OprD, responsible for imipenem uptake. Cells with low c-di-GMP levels are consequently more sensitive to this antibiotic. Main efflux pumps or β-lactamase genes did not show altered mRNA levels in P. aeruginosa strains with modified different c-di-GMP concentrations. Together, our findings show that c-di-GMP levels modulate fitness of planktonic cultures in the presence of imipenem.
| Type de document: | Article |
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| Mots-clés libres: | . |
| Centre: | Centre INRS-Institut Armand Frappier |
| Date de dépôt: | 29 avr. 2014 18:54 |
| Dernière modification: | 22 déc. 2016 19:23 |
| URI: | https://espace.inrs.ca/id/eprint/2181 |
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