Tremblay, Anne-Geneviève; Kharrat, Fatma et Vaillancourt, Cathy 
ORCID: https://orcid.org/0000-0003-0543-6244
(2025).
Melatonin, a multi-weaponed soldier: effect of oxidative stress in trophoblast cells
In: IFPA 2025, 17th-20th September 2020, Erfurt-Jena, Germany.
Résumé
Objectives: Melatonin is a powerful antioxidant that is produced by and
protects healthy trophoblastic cells from oxidative stress. On the other
hand, in choriocarcinoma cells, this indolamine promotes cell death, but
the mechanism behind this effect remains to be studied. The main
objective was to determine the effect of melatonin on human BeWo
choriocarcinoma cell line by evaluating its effect on oxidative stress
markers and its antioxidant defence enzymes.
Methods: BeWo cells were exposed under normoxia (8% O2) or hypoxiareoxygenation (H/R : 0.5% O2) to melatonin (1 nM, 1 uM and 1 mM).
Reactive oxygen species (ROS) levels were measured by the CarboxyDCFDA assay and enzyme antioxidant expression and activity was
analyzed by immunoblot and by colorimetry assays respectively. Lipid
peroxidation was analyzed by the thiobarbituric acid test (TBARS) as well
as by ELISA and protein carbonylation by fluorimetry. The apoptotic
marker caspase 8 was assessed by western blot.
Results: Melatonin increases ROS levels (P < 0.0001) and Xantine Oxidase
(XO) expression (P < 0,05), but decreases the activity of superoxide dismutases (SODs) (1μM; P < 0,05), glutathione peroxidases (GPx), (1 μM and
1nM; P < 0,05) and catalase (CAT) (1μM P < 0,01) compared with vehicle
control (dimethylsufoxide, DMSO) in BeWo cells. Melatonin also increased
lipid peroxidation and protein carbonylation (1μM and 1 nM ;P < 0,05) in
BeWo cells as well as the cleavage of caspase 8 compared with vehicle
control (DMSO) in BeWo cells.
Conclusion: Our results show that melatonin increases oxidative stress in
placental choriocarcinoma cells, suggesting an anti-tumour effect for this
indolamine. These findings could help generate a more complete and
effective approach to cancer treatments.
| Type de document: | Document issu d'une conférence ou d'un atelier |
|---|---|
| Informations complémentaires: | Affiche scientifique P2.69 Placenta (2025) 171 :e314-e315 https://ifpa2025.com/wp-content/uploads/2025/09/Abstract-Book_IFPA2025-1.pdf |
| Mots-clés libres: | - |
| Centre: | Centre INRS-Institut Armand Frappier |
| Date de dépôt: | 16 juin 2026 14:56 |
| Dernière modification: | 16 juin 2026 14:56 |
| URI: | https://espace.inrs.ca/id/eprint/16796 |
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