Plant miRNAs influence soil bacterial growth and amino acid uptake, restructuring community composition

Dozois, Jessica; Duchesne, Marc-Antoine; Hallaf, Katel; Tremblay, Julien et Yergeau, Étienne ORCID: https://orcid.org/0000-0002-7112-3425 (2025). Plant miRNAs influence soil bacterial growth and amino acid uptake, restructuring community composition ISME Communications , vol. 5 , nº 1. pp. 1-14. DOI: 10.1093/ismeco/ycaf206.

Prévisualisation

PDF - Version publiée Disponible sous licence Creative Commons Attribution. Télécharger (1MB) | Prévisualisation

Résumé

Plants and microbes use many strategies to acquire soil amino acids. Recent findings suggest that genes related to amino acid metabolism and transport are influenced by plant miRNAs. Here, we first show that Arabidopsis modifies its root miRNA content when fertilized with a mixture of 17 amino acids. The miRNAs that responded to amino acid fertilization and other rhizosphere-abundant miRNAs were applied to a simplified soil community, grown with diverse amino acid sources, to test if they interfered with microbial community growth, community composition, and amino acid consumption. Plant miRNAs affected the community’s growth in over 70% of the amino acid sources. The impact of plant miRNAs also depended on the N source supplied to the microbial community, with the strongest effect observed with L-lysine. Specifically, ath-miR159a reduced the microbial consumption of L-lysine, further supporting that plant miRNAs can influence microbial amino acid uptake. Plant miRNAs also strongly affected the relative abundance of specific bacterial taxa, which we subsequently isolated. These community shifts were explained by the subtle but robust impact of plant miRNAs on isolates' growth and, for two out of three isolates, on amino acid consumption. Surprisingly, while plant miRNAs inhibited amino acid consumption at both the community and isolate levels, the effects of plant miRNAs were mostly positive. Our results suggest that rhizospheric plant miRNAs might have a role in modulating the amino acid consumption of soil bacteria which reshapes the community, but not necessarily in a competitive framework.

Type de document:	Article
Informations complémentaires:	document ycaf206 Jessica Dozois was supported by a Tri-Agency Vanier Scholarship (grant CGV 180794) and two Fonds de recherche du Québec scholarships (https://doi.org/10.69777/297550 and https://doi. org/10.69777/281523). The research was funded by a National Research Council of Canada Ideation New Beginnings Grant, a NSERC Discovery Grant (RGPIN-2020-05723) and the Canada Research Chair in Plant Microbiome Engineering awarded to Etienne Yergeau (CRC-2023-00313)
Mots-clés libres:	Amino acids; nitrogen; plant miRNAs; plant-microbe interactions; soil bacterial communities
Centre:	Centre INRS-Institut Armand Frappier
Date de dépôt:	16 juin 2026 14:57
Dernière modification:	16 juin 2026 14:57
URI:	https://espace.inrs.ca/id/eprint/16788

Gestion Actions (Identification requise)

Modifier la notice