Protocol to assess changes in mitochondrial respiration in living cultured cells infected with flaviviruses

Barragan Torres, Viviana Andrea et Chatel-Chaix, Laurent ORCID: https://orcid.org/0000-0002-7390-8250 (2025). Protocol to assess changes in mitochondrial respiration in living cultured cells infected with flaviviruses STAR Protocols , vol. 6 , nº 3. pp. 1-20. DOI: 10.1016/j.xpro.2025.104051.

Prévisualisation

PDF - Version publiée Disponible sous licence Creative Commons Attribution. Télécharger (11MB) | Prévisualisation

Résumé

Mitochondria are essential organelles involved in energy production, making them prime targets for flaviviruses, such as dengue and Zika viruses, to enhance viral replication. Here, we present a protocol to measure multiple respiratory parameters in living hepatocarcinoma cells infected with flaviviruses in combination with pharmacological treatments or genetic expression modulation. We detail steps for infecting cells with flaviviruses and measuring the oxygen consumption rates using Seahorse technology. For complete details on the use and execution of this protocol, please refer to iScience. 2025;28 doi: 10.1016/j.isci.2024.111599

Type de document:	Article
Informations complémentaires:	L.C.-C. is receiving a Research Scholar (Senior) salary support from Fonds de Recherche du Québec-Sante´ (FRQS; https:// doi.org/10.69777/330358). The Seahorse analyzer was acquired through a young investigator infrastructure grant from the Armand-Frappier Foundation and Institut National de la Recherche Scientifique to L.C.-C. This study was supported by project grants from the Canadian Institutes of Health Research (CIHR; PJT153020 and PJT470746), awarded to L.C.-C.
Mots-clés libres:	Cell-based Assays; Metabolism; Microbiology
Centre:	Centre INRS-Institut Armand Frappier
Date de dépôt:	08 juin 2026 20:13
Dernière modification:	08 juin 2026 20:13
URI:	https://espace.inrs.ca/id/eprint/16645

Gestion Actions (Identification requise)

Modifier la notice