Optimizing measurement of soil potential high-affinity H2 uptake activity across pH

Hou, Lijun; Constant, Philippe ORCID: https://orcid.org/0000-0003-2739-2801 et Whalen, Joann (2025). Optimizing measurement of soil potential high-affinity H2 uptake activity across pH Soil Science Society of American Journal , vol. 89 , nº 3. pp. 1-9. DOI: 10.1002/saj2.70072.

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Résumé

Soil high-affinity H2 uptake activity can be affected by many factors, including the soil pH. However, the method to determine how pH affects high-affinity H2 uptake activity should be updated. The effect of pH on the biological high-affinity H2 uptake in agricultural soils was compared using three pH buffer systems in the pH 4-8 range. Soil pH was adjusted to the target pH using a buffer system (1 g soil/5 mL pH buffer). Soil slurries were treated with heat (autoclaving) or a chemical (25% v/w of toluene addition, microbial inhibitor) to inhibit biological activity. Sterile pH buffer was used as a negative control. The sterile soil slurry (heat sterilization) was the optimal reference control for measuring biological high-affinity H2 uptake activity. Biological H2 uptake activity was resistant to toluene, particularly at extreme pH levels. Overall, soil pH (p = 0.95) and pH buffer systems (p = 0.46) did not affect the high-affinity H2 uptake activity in the tested agricultural soils. We provide an updated method to accurately measure the potential high-affinity H2 uptake activity in soil, with an emphasis on the importance of controlling the soil pH.

Type de document:	Article
Informations complémentaires:	Fonds de recherche du Québec – Nature et technologies, Grant/Award Number: 2021-PR-298015; China Scholarship Council, Grant/Award Number: 202006300027; Mitacs, Grant/Award Number: FR49697
Mots-clés libres:	-
Centre:	Centre INRS-Institut Armand Frappier
Date de dépôt:	08 juin 2026 20:12
Dernière modification:	08 juin 2026 20:12
URI:	https://espace.inrs.ca/id/eprint/16643

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