Chavan, Shraddha; Yadav, Bhoomika; Tyagi, Rajeshwar Dayal; Wong, Jonathan Woon-Chung et Drogui, Patrick 
ORCID: https://orcid.org/0000-0002-3802-2729
(2024).
Comparative study of chemical and enzymatic pre-treatments of Kitchen waste (KW) to generate fermentable sugars for the production of polyhydroxyalkanoates (PHA).
Biochemical Engineering Journal
, vol. 204
.
p. 109240.
DOI: 10.1016/j.bej.2024.109240.
Résumé
Kitchen waste (KW) production is anticipated to rise because of accelerated urban and economic growth, particularly in Asian nations. Therefore, applications/recycling of KW should be explored for its effective utilization to generate a sustainable source of energy that will ultimately avoid massive economic and energy losses. The main goal of this study was to investigate the combinational effect of thermochemical and enzymatic pre-treatment on synthetic KW to enhance the release of fermentable sugars for the synthesis of value-added products such as polyhydroxyalkanoates (PHAs). The thermochemical pre-treatment of KW samples was performed using 4N HCl, 4N H₂SO₄, and 4N NaOH at a concentration of 0–30% (v/v), 30–100 °C for 0–120 min. Furthermore, to enhance the total fermentable sugars, variable enzyme dosages were added after acidic or alkaline pre-treatments (0–1% v/v α-amylase and 0–1% v/v amyloglucosidase-AMG) while maintaining a constant pH 5, temperature 50 °C, and time 30 min. Pre-treatment of KW with acid (15% v/v 4 N HCl in water) at temperature 100 °C, followed by enzymatic hydrolysis, i.e., 0.6% (v/v) α-amylase and 0.4% (v/v) amyloglucosidase (at pH 5, 50 0C and 60 min) resulted in the maximum concentration of fermentable reducing sugars (46.13 g/L) in the hydrolysate.
| Type de document: | Article |
|---|---|
| Mots-clés libres: | kitchen waste; pre-treatment; fermentation; bioplastics; amylase enzyme; amyloglucosidase; reducing sugars |
| Centre: | Centre Eau Terre Environnement |
| Date de dépôt: | 09 juill. 2024 18:12 |
| Dernière modification: | 09 juill. 2024 18:12 |
| URI: | https://espace.inrs.ca/id/eprint/15497 |
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