Sonier, Brigitte; Arseneault, Madeleine; Lavigne, Carole; Ouellette, Rodney J. et Vaillancourt, Cathy 
ORCID: https://orcid.org/0000-0003-0543-6244
(2006).
The 5-HT2A serotoninergic receptor is expressed in the MCF-7 human breast cancer cell line and reveals a mitogenic effect of serotonin
Biochemical and Biophysical Research Communications
, vol. 343
, nº 4.
pp. 1053-1059.
DOI: 10.1016/j.bbrc.2006.03.080.
Résumé
Serotonin (5-hydroxytryptamine, 5-HT) has been described as a mitogen in a variety of cell types and carcinomas. It exerts its mitogenic effect by interacting with a wide range of 5-HT receptor types. Certain studies suggest that some selective serotonin re-uptake inhibitors promote breast cancer in animals and humans. This study attempts to clarify the role of serotonin in promoting the growth of neoplastic mammary cells. Expression of the 5-HT(2A) serotoninergic receptor subtype in MCF-7 cells was determined by RT-PCR, Western blotting, and immunofluorescence analysis. The mitogenic effect of 5-HT on MCF-7 cells was determined by means of the MTT proliferation assay. We have demonstrated that the 5-HT(2A) receptor subtype is fully expressed in the MCF-7 human breast cancer cell line, in terms of encoding mRNA and receptor protein. Automated sequencing has confirmed that the 5-HT(2A) receptor present in this cell line is identical to the 5-HT(2A) receptor found in human platelets and in human cerebral cortex. Furthermore, this receptor was found by immunofluorescence to be on the plasma membrane. MTT proliferation assays revealed that 5-HT and DOI, a selective 5-HT(2A) receptor subtype agonist, stimulated MCF-7 cell. These results indicate that 5-HT plays a mitogenic role in neoplastic mammary cells. Our data also indicate that 5-HT exerts this positive growth effect on MCF-7 cells through, in part, the 5-HT(2A) receptor subtype, which is fully expressed in this cell line.
| Type de document: | Article |
|---|---|
| Mots-clés libres: | 5-HT2A-receptors; Breast cancer; MCF-7 cells; Proliferation; Serotonin |
| Centre: | Centre INRS-Institut Armand Frappier |
| Date de dépôt: | 05 nov. 2024 21:48 |
| Dernière modification: | 05 nov. 2024 21:48 |
| URI: | https://espace.inrs.ca/id/eprint/15177 |
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