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Malarial hemozoin activates the NLRP3 inflammasome through Lyn and Syk kinases.


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Shio, Marina Tiemi; Eisenbarth, Stephanie C.; Savaria, Myriam; Vinet, Adrien F.; Bellemare, Marie-Josée; Harder, Kenneth W.; Sutterwala, Fayyaz S.; Bohle, D. Scott; Descoteaux, Albert ORCID logoORCID: https://orcid.org/0000-0002-0633-5309; Flavell, Richard A. et Olivier, Martin (2009). Malarial hemozoin activates the NLRP3 inflammasome through Lyn and Syk kinases. PLoS Pathogens , vol. 5 , nº 8. pp. 1-14. DOI: 10.1371/journal.ppat.1000559.

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The intraerythrocytic parasite Plasmodium-the causative agent of malaria-produces an inorganic crystal called hemozoin (Hz) during the heme detoxification process, which is released into the circulation during erythrocyte lysis. Hz is rapidly ingested by phagocytes and induces the production of several pro-inflammatory mediators such as interleukin-1 beta (IL-1 beta). However, the mechanism regulating Hz recognition and IL-1 beta maturation has not been identified. Here, we show that Hz induces IL-1 beta production. Using knockout mice, we showed that Hz-induced IL-1 beta and inflammation are dependent on NOD-like receptor containing pyrin domain 3 (NLRP3), ASC and caspase-1, but not NLRC4 (NLR containing CARD domain). Furthermore, the absence of NLRP3 or IL-1 beta augmented survival to malaria caused by P. chabaudi adami DS. Although much has been discovered regarding the NLRP3 inflammasome induction, the mechanism whereby this intracellular multimolecular complex is activated remains unclear. We further demonstrate, using pharmacological and genetic intervention, that the tyrosine kinases Syk and Lyn play a critical role in activation of this inflammasome. These findings not only identify one way by which the immune system is alerted to malarial infection but also are one of the first to suggest a role for tyrosine kinase signaling pathways in regulation of the NLRP3 inflammasome.

Type de document: Article
Informations complémentaires: document e1000559
Mots-clés libres: -
Centre: Centre INRS-Institut Armand Frappier
Date de dépôt: 09 juill. 2024 15:10
Dernière modification: 09 juill. 2024 15:10
URI: https://espace.inrs.ca/id/eprint/15165

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