Ross, Nathalie; Villemur, Richard 
ORCID: https://orcid.org/0000-0001-9768-8937; Deschênes, Louise et Samson, Réjean
(2001).
Clogging of a limestone fracture by stimulating groundwater microbes
Water Research
, vol. 35
, nº 8.
pp. 2029-2037.
DOI: 10.1016/s0043-1354(00)00476-0.
Résumé
Biological clogging is promoted in aquifers either to contain or to remediate groundwater. In this study. an apparatus able to detect small changes in hydraulic conductivity (K) was developed to measure the clogging of a single fracture in limestone, following microbial stimulation. The fracture had a 2.5 mm(2) section and was 50cm long. Prior to the inoculation of the limestone, the sequencing ol representative clones from 16S rRNA gents isolated from groundwater, showed significant affiliation with Cytophaga spp., Arcobacter spp. and Rhizobium spp. These bacteria are known to secrete extracellular polymeric substances and form biofilms. When nutrients were added to the inoculated limestone, a decrease in K occurred after 8 days, reaching 0.8% L of its initial value after 22 days (K-n = 330 cm min(-1)) This study should that a stimulation of Indigenous microbes from groundwater effectively clogged a macrofracture ill limestone. suggesting the potential application of biobarriers in Fractured rock aquifers.
| Type de document: | Article |
|---|---|
| Mots-clés libres: | fractured rock; groundwater; indigenous micro-organisms; clogging; biofilm; extracellular polymeric substances |
| Centre: | Centre INRS-Institut Armand Frappier |
| Date de dépôt: | 14 avr. 2025 15:33 |
| Dernière modification: | 14 avr. 2025 15:33 |
| URI: | https://espace.inrs.ca/id/eprint/15121 |
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