Le-Tien, Canh; Lafortune, Roxanne; Shareck, Francois et Lacroix, Monique 
ORCID: https://orcid.org/0000-0002-2042-4033
(2007).
DNA analysis of a radiotolerant bacterium Pantoea agglomerans by FT-IR spectroscopy
Talanta
, vol. 71
, nº 5.
pp. 1969-1975.
DOI: 10.1016/j.talanta.2006.09.003.
Résumé
A radiation tolerance strain, Pantoea agglomerans was isolated from gamma-irradiated carrot samples (Daucus carota). D-10 determination showed that the radioresistance of this bacterium is five-fold higher than Escherichia coli, both belonging to the family of Enterobacteriaceae. DNA isolated from untreated and irradiated bacteria] cells was analyzed by FT-IR spectroscopy to investigate the radiotolerance of this bacterium. At doses < 5 kGy, an alteration of the interbase hydrogen networks was observed and characterized mainly by an increase of bands assigned to the carbonyl non-pairing and the free amine groups. Moderate breakage of the DNA backbone and damage of the osidic structure were also observed. Similar spectral profiles were noticed at doses >= 5 kGy, but additional increase of the band intensity of C=C and C=N suggests damages of nucleobases. High number of asymmetric PO2- and upper shift of symmetric PO2- are indicative of DNA strand breaks. Osidic damages were evidenced by decrease of the absorption bands ascribed to deoxyribosyl moieties and by appearance of C-OH band. DNA degradation at high irradiation doses was also noticed by electrophoresis using agarose gel. It appeared that DNA underwent covalent cross-linking, as revealed by agglomeration of DNA in the wells of agarose gel.
| Type de document: | Article |
|---|---|
| Mots-clés libres: | Radiotolerance; DNA; γ-Irradiation; Pantoea agglomerans; FT-IR; Electrophoresis |
| Centre: | Centre INRS-Institut Armand Frappier |
| Date de dépôt: | 04 nov. 2024 20:24 |
| Dernière modification: | 04 nov. 2024 20:24 |
| URI: | https://espace.inrs.ca/id/eprint/14897 |
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