Isabelle, Martine; Villemur, Richard 
ORCID: https://orcid.org/0000-0001-9768-8937; Juteau, Pierre et Lépine, François
(2011).
Isolation of estrogen-degrading bacteria from an activated sludge bioreactor treating swine waste, including a strain that converts estrone to beta-estradiol
Canadian Journal of Microbiology
, vol. 57
, nº 7.
pp. 559-568.
DOI: 10.1139/w11-051.
Résumé
An estrogen-degrading bacterial consortium from a swine wastewater biotreatment was enriched in the presence of low concentrations (1 mg/L) of estrone (E1), 17 beta-estradiol (beta E2), and equol (EQO) as sole carbon sources. The consortium removed 99% +/- 1% of these three estrogens in 48 h. Estrogen removal occurred even in the presence of an ammonia monooxygenase inhibitor, suggesting that nitrifiers are not involved. Five strains showing estrogen-metabolizing activity were isolated from the consortium on mineral agar medium with estrogens as sole carbon source. They are related to four genera (Methylobacterium (strain M16.1R), Ochrobactrum (strains MI6.1B and MI9.3), Pseudomonas (strain MI14.1), and Mycobacterium (strain MI21.2)) distributed among three classes (Alphaproteobacteria, Gammaproteobacteria, and Actinobacteria). Depending on the culture medium, strains MI6.1B, MI9.3, MI14.1, and MI21.2 partially transform beta E2 into E1, whereas Methylobacterium sp. strain MI6.1R reduces E1 into beta E2 under aerobic conditions, in contrast with the usually observed conversion of beta E2 into E1. Since beta E2 is a more potent endocrine disruptor than E1, it means that the presence of Methylobacterium sp. strain MI6.1R (or other bacteria with the same E1-reducing activity) in a treatment could transiently increase the estrogenicity of the effluent. MI6.1R can also reduce the ketone group of 16-ketoestradiol, a hydroxylated analog of E1. All beta E2 and E1 transformation activities were constitutive, and many of them are favoured in a rich medium than a medium containing no other carbon source. None of the isolated strains could degrade EQO.
| Type de document: | Article |
|---|---|
| Mots-clés libres: | - |
| Centre: | Centre INRS-Institut Armand Frappier |
| Date de dépôt: | 25 mars 2024 15:13 |
| Dernière modification: | 25 mars 2024 15:13 |
| URI: | https://espace.inrs.ca/id/eprint/14769 |
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