Dépôt numérique
RECHERCHER

Homeostatic interplay between bacterial cell-cell signaling and iron in virulence

Téléchargements

Téléchargements par mois depuis la dernière année

Hazan, Ronen; He, Jianxin; Xiao, Gaoping; Dekimpe, Valérie; Apidianakis, Yiorgos; Lesic, Biliana; Astrakas, Christos; Déziel, Éric ORCID logoORCID: https://orcid.org/0000-0002-4609-0115; Lépine, François et Rahme, Laurence G. (2010). Homeostatic interplay between bacterial cell-cell signaling and iron in virulence PLoS Pathogens , vol. 6 , nº 3. pp. 1-14. DOI: 10.1371/journal.ppat.1000810.

[thumbnail of Homeostatic Interplay between Bacterial Cell-Cell Signaling and Iron in Virulence.pdf]
Prévisualisation
PDF - Version publiée
Disponible sous licence Creative Commons Attribution.

Télécharger (1MB) | Prévisualisation

Résumé


Pathogenic bacteria use interconnected multi-layered regulatory networks, such as quorum sensing (QS) networks to sense and respond to environmental cues and external and internal bacterial cell signals, and thereby adapt to and exploit target hosts. Despite the many advances that have been made in understanding QS regulation, little is known regarding how these inputs are integrated and processed in the context of multi-layered QS regulatory networks. Here we report the examination of the Pseudomonas aeruginosa QS 4-hydroxy-2-alkylquinolines (HAQs) MvfR regulatory network and determination of its interaction with the QS acyl-homoserine-lactone (AHL) RhlR network. The aim of this work was to elucidate paradigmatically the complex relationships between multi-layered regulatory QS circuitries, their signaling molecules, and the environmental cues to which they respond. Our findings revealed positive and negative homeostatic regulatory loops that fine-tune the MvfR regulon via a multi-layered dependent homeostatic regulation of the cell-cell signaling molecules PQS and HHQ, and interplay between these molecules and iron. We discovered that the MvfR regulon component PqsE is a key mediator in orchestrating this homeostatic regulation, and in establishing a connection to the QS rhlR system in cooperation with RhlR. Our results show that P. aeruginosa modulates the intensity of its virulence response, at least in part, through this multi-layered interplay. Our findings underscore the importance of the homeostatic interplay that balances competition within and between QS systems via cell-cell signaling molecules and environmental cues in the control of virulence gene expression. Elucidation of the fine-tuning of this complex relationship offers novel insights into the regulation of these systems and may inform strategies designed to limit infections caused by P. aeruginosa and related human pathogens.

Type de document: Article
Informations complémentaires: document e1000810
Mots-clés libres: -
Centre: Centre INRS-Institut Armand Frappier
Date de dépôt: 09 juill. 2024 14:11
Dernière modification: 09 juill. 2024 14:56
URI: https://espace.inrs.ca/id/eprint/14746

Gestion Actions (Identification requise)

Modifier la notice Modifier la notice