Dépôt numérique

Increased galectin-7 gene expression in lymphoma cells is under the control of DNA methylation

Demers, Mélanie; Couillard, Julie; Giglia-Mari, Giuseppina; Magnaldo, Thierry et St-Pierre, Yves ORCID logoORCID: https://orcid.org/0000-0002-1948-2041 (2009). Increased galectin-7 gene expression in lymphoma cells is under the control of DNA methylation Biochemical and Biophysical Research Communications , vol. 387 , nº 3. pp. 425-429. DOI: 10.1016/j.bbrc.2009.07.015.

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Recent studies have reported that elevated levels of galectin-7 in different types of cancer. The mechanisms underlying its abnormal regulation in cancer cells remain, however, unknown. Here, we have examined the relationship between galectin-7 and p53, a gene previously associated with upregulation of galectin-7. While RNA and protein analyses revealed a consistent and irreversible upregulation of galectin-7 throughout progression of lymphoma, no correlation with p53 was found. In fact, most of the lymphoma cell lines expressing high levels of galectin-7 did not express any detectable level of p53, although expressed p21Waf1/Cip1 gene following doxorubicin treatment, indicating that p53 was functional in these cells. Methylation-specific polymerase chain reaction (MS-PCR) analyses rather suggested that galectin-7 expression was associated with changes in DNA methylation. This conclusion was supported by data using demethylating agent 5-aza-dC. Furthermore, disruption of the DNA methylases dnmt1 and dnmt3a induced galectin-7. Collectively, our data suggest that abnormal expression of galectin-7 in lymphoma cells is not dependent on p53, but is rather associated with DNA hypomethylation.

Type de document: Article
Mots-clés libres: Galectin-7; Lymphoma; p53; DNA methylation
Centre: Centre INRS-Institut Armand Frappier
Date de dépôt: 29 juin 2024 21:43
Dernière modification: 29 juin 2024 21:43
URI: https://espace.inrs.ca/id/eprint/14577

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