Dépôt numérique

Burn injury induces the expression of cystine/glutamate transporter (xc-) in mouse T cells

D'Elia, Michele; Patenaude, Julie; Dupras, Charles et Bernier, Jacques ORCID logoORCID: https://orcid.org/0000-0002-0594-5922 (2009). Burn injury induces the expression of cystine/glutamate transporter (xc-) in mouse T cells Immunology Letters , vol. 125 , nº 2. pp. 137-144. DOI: 10.1016/j.imlet.2009.06.011.

Ce document n'est pas hébergé sur EspaceINRS.


System xc- transporter, formed by the association of CD98 and xCT proteins, regulates the import of cystine into cells and is poorly expressed in T lymphocytes. Thermal injury is associated with high oxidative stress, decreased levels of glutathione (GSH) and protein deficiency, all described as promoters of xCT expression and system xc- activity. T cell dysfunction is a consequence of thermal injury and has been related to oxidative stress. In order to evaluate if thermal injury induced system xc- expression in splenic T lymphocytes, cells were isolated from sham- and burn-injured mice at day 10 post-burn and cultured in 2-mercaptoethanol (2-ME)-rich and -free media. Isolated splenic T cells were stimulated and cell proliferation, system xc- expression and cystine transport activity were measured. Our results demonstrate that only burn-injured T cells express xCT and proliferate in (2-ME)-free media. In these cells, viability and CD25 expression was higher than control T cells. xc- system expression was responsible for significantly higher 14C-cystine uptake by burn-injured T cells and its inhibition by sulfasalazine (SASP) decreased significantly their proliferation. Overall, these results demonstrate that xCT expression is induced by thermal injury in T lymphocytes and that cystine import by xc- leads to T cell dysfunction.

Type de document: Article
Mots-clés libres: T cells; Spleen; Inflammation; Cell proliferation; Mice; Burn injury
Centre: Centre INRS-Institut Armand Frappier
Date de dépôt: 29 juin 2024 21:41
Dernière modification: 29 juin 2024 21:41
URI: https://espace.inrs.ca/id/eprint/14571

Gestion Actions (Identification requise)

Modifier la notice Modifier la notice