Bisaillon, Ariane; Beaudet, Rejean; Lépine, François; Déziel, Éric ORCID: https://orcid.org/0000-0002-4609-0115 et Villemur, Richard
ORCID: https://orcid.org/0000-0001-9768-8937
(2010).
Identification and characterization of a novel CprA reductive dehalogenase specific to highly chlorinated phenols from Desulfitobacterium hafniense strain PCP-1
Applied and Environmental Microbiology
, vol. 76
, nº 22.
pp. 7536-7540.
DOI: 10.1128/AEM.01362-10.
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Résumé
Desulfitobacterium hafniense strain PCP-1 reductively dechlorinates pentachlorophenol (PCP) to 3-chlorophenol and a variety of halogenated arom. compds. at the ortho, meta, and para positions. Several reductive dehalogenases (RDases) are thought to be involved in this cascade of dehalogenation. We partially purified a novel RDase involved in the dechlorination of highly chlorinated phenols from strain PCP-1 cultivated in the presence of 2,4,6-trichlorophenol. The RDase was membrane assocd., and the activity was sensitive to oxygen, with a half-life of 128 min upon exposure to air. The pH and temp. optima were 7.0 and 55°C, resp. Several highly chlorinated phenols were dechlorinated at the ortho positions. The highest dechlorinating activity levels were obsd. with PCP, 2,3,4,5-tetrachlorophenol, and 2,3,4-trichlorophenol. 3-Chloro-4-hydroxyphenylacetate, 3-chloro-4-hydroxybenzoate, dichlorophenols, and monochlorophenols were not dechlorinated. The apparent Km value for PCP was 46.7 μM at a Me viologen concn. of 2 mM. A mixt. of iodopropane and titanium citrate caused a light-reversible inhibition of the dechlorinating activity, suggesting the involvement of a corrinoid cofactor. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the partially purified prepn. revealed 2 bands with apparent mol. masses of 42 and 47 kDa. Mass spectrometry anal. using Mascot to search the genome sequence of D. hafniense strain DCB-2 identified the 42-kDa band as NADH-quinone oxidoreductase, subunit D, and the 47-kDa band as the putative chlorophenol RDase CprA3. This is the first report of an RDase with high affinity and high dechlorinating activity toward PCP. [on SciFinder(R)]
Type de document: | Article |
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Mots-clés libres: | - |
Centre: | Centre INRS-Institut Armand Frappier |
Date de dépôt: | 29 juin 2024 20:46 |
Dernière modification: | 29 juin 2024 20:46 |
URI: | https://espace.inrs.ca/id/eprint/14422 |
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