Dépôt numérique

Human term villous trophoblasts synthesize melatonin and express its receptors

Lanoix, Dave et Vaillancourt, Cathy ORCID logoORCID: https://orcid.org/0000-0003-0543-6244 (2007). Human term villous trophoblasts synthesize melatonin and express its receptors In: 13th International-Federation-of-Placenta-Association Meeting, August 17-22, 2007, Kinston, Canada.

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Objectives: Melatonin concentrations increase in maternal blood during pregnancy, reaching a maximum at term. Concentrations are lower in case of pre-eclampsia and intrauterine growth restriction compared to normal pregnancies. We previously demonstrated that melatonin regulates the secretion of human chorionic gonadotropin via its receptors in JEG-3 and BeWo cell lines, both in vitro models of human trophoblast. To date, human placenta’s ability to synthesise melatonin de novo and the role of melatonin in placental functions have not been elucidated. Therefore, we evaluated the expression and activity of melatonin synthesizing enzymes in human term villous trophoblast and characterized the expression of the MT1, MT2 and RORa1 melatonin receptors. Methods: Expression of arylalkylamine-N-acetyltransferase (AANAT) and hydroxyindolO-methyltransferase (HIOMT) melatonin synthesizing enzymes in addition to MT1, MT2 and RORa1 melatonin receptors was measured by RT-PCR and western blotting in villous cytotrophoblast (vCTB) and syncytiotrophoblast (STB) from human term placenta primary cultures and by immunohistochemistry in human term placental tissue. Enzymatic activity of AANAT and HIOMT was assessed by radiometric assays. Results: AANAT and HIOMT melatonin synthesizing enzymes are expressed and active in human term vCTB, STB and placental tissue. Furthermore, MT1, MT2 and RORa1 melatonin receptors are expressed in human term vCTB and STB. Conclusions: These results demonstrate, for the first time, that human term villous trophoblasts synthesize melatonin and express its receptors, suggesting a possible autocrine/paracrine function for melatonin in human placenta.

Type de document: Document issu d'une conférence ou d'un atelier
Informations complémentaires: Affiche scientifique A8.03 Placenta (2007) 28(8-9):A71
Mots-clés libres: -
Centre: Centre INRS-Institut Armand Frappier
Date de dépôt: 30 mars 2024 22:09
Dernière modification: 30 mars 2024 22:09
URI: https://espace.inrs.ca/id/eprint/14296

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