Dépôt numérique

Expression and function of melatonine receptors in human placenta and choriocarcinoma cell lines

Lanoix, Dave et Vaillancourt, Cathy ORCID logoORCID: https://orcid.org/0000-0003-0543-6244 (2006). Expression and function of melatonine receptors in human placenta and choriocarcinoma cell lines In: 12th Meeting of the International-Federation-of-Placenta-Association, September 06-09, 2006, Kobe, Japan.

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Background: Melatonin crosses the placenta and enters the fetal circulation. Moreover, experimental data suggest a possible influence of melatonin on placental function and fetal development in human. To date, the expression and role of melatonin receptors in human placenta choriocarcinoma cell lines and in human term placental tissue remain to be elucidated.

Methods and Results: Results from RT-PCR, Western blotting, immununohistochemis- try and confocal microscopy demonstrated that the MT1, MT2 and RORαl melatonin re- ceptors are expressed in the human term placental tissue and in choriocarcinoma cell lines JEG-3 and BeWo. Furthermore, enzyme-linked immunosorbent assay (ELISA) showed that 6-chloromelatonin inhibits, in a dose-dependant manner, forskolin-stimulated human chorionic gonadotrophin-β (hCG-β) secretion but had no effect on basal hCG-β levels, in JEG-3 and BeWo cells. This effect of 6-chloromelatonin on forskolin-stimulated hCG-β secretion was abolished by pertussis toxin suggesting that melatonin regulates hCG-β production by an action involving an inhibitory Gi/o-protein.

Conclusion: These results demonstrate, for the first time, the expression of melatonin receptors in human term placental tissues and in choriocarcinoma cells and suggest a possible paracrine/autocrine function for melatonin in human placenta.

Type de document: Document issu d'une conférence ou d'un atelier
Informations complémentaires: Affiche scientifique P80 Placenta (2006) 27(9-10):A38
Mots-clés libres: -
Centre: Centre INRS-Institut Armand Frappier
Date de dépôt: 30 mars 2024 22:48
Dernière modification: 30 mars 2024 22:48
URI: https://espace.inrs.ca/id/eprint/14295

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