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Clenbuterol: screening and confirmation

Ayotte, Christiane ORCID logoORCID: https://orcid.org/0000-0002-6352-2071 (1993). Clenbuterol: screening and confirmation In: 10th Cologne Workshop on Dope Analysis, 7th to 12th June 1992, June 7-12, 1992, Cologne, Allemagne.

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Clenbuterol is a ß2-agonist and a stimulant of the central nervous system; it is used as an antiasthmatic and tocolytic agent. However, it has been reported that Clenbuterol was being used as growth promoter in the cattle industy, bringing up the fact that it could also be an anabolic agent. Specialized Bodybuilding literature began to describe the use of Clenbuterol a few years ago and it appeared that the administration of this substance could be widespread among athletes seeking for muscle mass enhancement and strength. The review of analytical methods available for the identification of Clenbuterol showed that due to the low dose administered (20 to 80 gg/per day for the anti-asthma therapy), low limits of detection were needed to detect it (high picogram level/mL of urine). Since the TMS-derivatization was already used for the screening of adrogenic anabolic steroids, we established at first the limits of detection in the routine procedure IV. The mass spectrum of the di-TMS derivative (molecular ion at m/z 420) showed very characteristic ions at m/z 405 (-Me 335 (Ar0H+ ), 300 (ArOH-C1+) and 86 (base peak, Through normal procedure of derivatization (MSTFA:TMSI, dithioerythritol), Clenbuterol could be detected up to 2.5 ng/mL. These results were also confirmed by spiking urine samples with Clenbuterol hydrochloride. For confirmation purposes, the free fraction isolated from Sep-Pack C18 cartridges and diethyl ether extraction at pH 9 was used: the aliquot was concentrated four times by extracting twice the amount used in the screening procedure and then derivatized in a final volume of 50 tula instead of 100 ILL: the extraction recovery estimated analytically with reference to the injection of the standard was around 75% . Finally, an excretion study was performed with a single dose of 100 1.zg of Clenbuterol hydrochloride. Clenbuterol was identified in the urine samples up to the last collection time ie: 24 hours. Since November 1991, Clenbuterol has been identified in nine urine samples (mainly Body Builder' s).

Type de document: Document issu d'une conférence ou d'un atelier
Informations complémentaires: In: M. Donike, H. Geyer, A. Gotzmann, U. Mareck-Engelke, S. Rauth (eds.) 10th Cologne Workshop On Dope Analysis 7th to 12th June 1992. Sport und Buch Strauß, Köln, (1993) 185-196
Mots-clés libres: Analysis; Methods; Clenbuterol; Doping in sports; Detection; Confirmation; Mass Sprectrometry
Centre: Centre INRS-Institut Armand Frappier
Date de dépôt: 12 mars 2024 16:44
Dernière modification: 12 mars 2024 16:44
URI: https://espace.inrs.ca/id/eprint/14256

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