Dépôt numérique

Molecular evolutionary analysis of the Nramp family

Richer, Étienne; Courville, Pascal et Cellier, Mathieu ORCID logoORCID: https://orcid.org/0000-0002-6084-434X (2004). Molecular evolutionary analysis of the Nramp family In: The Nramp Family. Molecular Biology Intelligence Unit . Plenum, New York, pp. 178-194.

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A review. The natural resistance-assocd. macrophage protein (Nramp) belongs to a family of ion permeases highly conserved in eukaryotes that originated in prokaryotes. Three phylogenetic groups of homologs were identified in Bacteria based on functional genomic approaches. Proteins of group A were characterized as H+-dependent Manganese Transporter (MntH A) representing functional homologs of eukaryotic Nramp. Heterologous expression in E. coli of MntH B and C proteins modulated bacterial sensitivity to cobalt and cadmium, suggesting conserved function among MntH groups. Genome sequencing of model eukaryotes revealed two groups of eukaryotic Nramp. Conservation in invertebrates of a 'prototype' suggests early duplication of an ancestral Nramp gene. Family trees inferred from phylogenetic analyses using various approaches indicate successive divergence of MntH B, MntH A, eukaryotic Nramp and MntH C. MntH C shows high level sequence identity with 'prototype' Nramp, heterogeneous variation of a.a. replacement rate among sites, and predicted divergence from 'archetype' Nramp. The data suggest a relatively recent origin for MntH C, derived from an eukaryote 'prototype' Nramp gene. This proposition is discussed in the context of bacterial infection.

Type de document: Chapitre de livre
Mots-clés libres: -
Centre: Centre INRS-Institut Armand Frappier
Date de dépôt: 31 mars 2024 16:28
Dernière modification: 31 mars 2024 16:28
URI: https://espace.inrs.ca/id/eprint/14247

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