Dépôt numérique

Regulation of nitric oxide synthesis by beta-adrenergic receptors in the nuclear envelope

Vaniotis, George; Merlen, Clémence; Tadevosyan, Artavazd; Villeneuve, Louis R.; Chatenet, David ORCID logoORCID: https://orcid.org/0000-0002-7270-4328; Zamboni, Robert; Fournier, Alain; Hébert, Terence E et Allen, Bruce G. (2012). Regulation of nitric oxide synthesis by beta-adrenergic receptors in the nuclear envelope In: Experimental Biology Meeting, April 21-25, 2012, San Diego, California,.

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Both β-adrenergic receptors (β-ARs) and endothelin receptors (ETRs) have been shown to be present on nuclear membranes in adult cardiac ventricular myocytes. These nuclear-localized receptors are functional with respect to ligand binding and effector activation. Furthermore, isoproterenol (ISO) increases transcription initiation in isolated nuclei whereas endothelin-1 (ET-1) has the opposite effect. Here we investigate whether ISO and ET-1 regulate nitric oxide (NO) production in nuclear membranes. Isolated nuclei were incubated with a fluorescent NO indicator, 4,5-diaminofluorescein (DAF-2), followed by either ET-1 or ISO treatment. In both cases, an increase in DAF-2 fluorescence was observed: this increase was inhibited by the NO synthase inhibitor, L-NAME. Pertussis toxin treatment also increased DAF-2 fluorescence. The PKB inhibitor triciribine, which converts ISO from an activator of transcription to an inverse agonist, potentiated ISO-mediated DAF-2 fluorescence without affecting ET-1 signalling. These experiments were repeated in intact myocytes using caged analogues of ISO and ET-1, and in combination with 4,5-diaminofluorescein diacetate (DAF-2 DA), a cell-permeant form of DAF-2. Our results suggest the nuclear β-ARs and ETRs regulate NO synthesis.

Type de document: Document issu d'une conférence ou d'un atelier
Informations complémentaires: FASEB Journal 26 (suppl. 1)
Mots-clés libres: -
Centre: Centre INRS-Institut Armand Frappier
Date de dépôt: 28 janv. 2024 14:57
Dernière modification: 28 janv. 2024 14:57
URI: https://espace.inrs.ca/id/eprint/14135

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