Dépôt numérique

Cardiotoxin-I: An Unexpectedly Potent Insulinotropic Agent

Nguyen, Thi Tuyet Nhung; Folch, Benjamin; Létourneau, Myriam; Vaudry, David; Truong, Nam Hai; Doucet, Nicolas ORCID logoORCID: https://orcid.org/0000-0002-1952-9380; Chatenet, David ORCID logoORCID: https://orcid.org/0000-0002-7270-4328 et Fournier, Alain (2012). Cardiotoxin-I: An Unexpectedly Potent Insulinotropic Agent Chembiochem , vol. 13 , nº 12. pp. 1805-1812. DOI: 10.1002/cbic.201200081.

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Insulin secretion from pancreatic β-cells is a complex process, involving the integration and interaction of multiple external and internal stimuli, in which glucose plays a major role. Understanding the physiology leading to insulin release is a crucial step toward the identification of new targets. In this study, we evaluated the presence of insulinotropic metabolites in Naja kaouthia snake venom. Only one fraction, identified as cardiotoxin-I (CTX-I) was able to induce insulin secretion from INS-1E cells without affecting cell viability and integrity, as assessed by MTT and LDH assays. Interestingly, CTX-I was also able to stimulate insulin secretion from INS-1E cells even in the absence of glucose. Although cardiotoxins have been characterized as potent hemolytic agents and vasoconstrictors, CTX-I was unable to induce direct hemolysis of human erythrocytes or to induce potent vasoconstriction. As such, this newly identified insulin-releasing toxin will surely enrich the pool of existing tools to study β-cell physiology or even open a new therapeutic avenue.

Type de document: Article
Mots-clés libres: cardiotoxin-I; insulin; ion channels; peptides; structure–function relationships
Centre: Centre INRS-Institut Armand Frappier
Date de dépôt: 09 mars 2024 15:27
Dernière modification: 09 mars 2024 15:27
URI: https://espace.inrs.ca/id/eprint/14068

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