Dépôt numérique

Stability of reference proteins in human placenta: General protein stains are the benchmark

Lanoix, Dave; St-Pierre, Joey; Lacasse, Andrée Anne; Viau, Melanie; Lafond, Julie et Vaillancourt, Cathy ORCID logoORCID: https://orcid.org/0000-0003-0543-6244 (2012). Stability of reference proteins in human placenta: General protein stains are the benchmark Placenta , vol. 33 , nº 3. pp. 151-156. DOI: 10.1016/j.placenta.2011.12.008.

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The stability of reference proteins in semi-quantitative Western blot experiments in normal and diseased placenta has never been studied. This study aims to determine the stability of five reference proteins and two general protein stains in placentas from preeclampsia, gestational diabetes mellitus and matched control pregnancies. The stability of the reference proteins was analysed using indicators of inter-group (P value) and intra-group (coefficient of variation) stability. The effect of different normalization strategies was determined by normalizing serotonin transporter (SERT) expression against the different reference protein markers. Results show significant expression variability of β-actin, glyceraldehyde 3-phosphate dehydrogenase (GAPDH), hypoxanthine phosphoribosyltransferase 1 (HPRT1), peptidylprolyl isomerase A (PPIA) and α-tubulin, and that amido black staining is the most stable reference protein marker. Furthermore, results show that SERT expression significantly differs according to the reference protein markers used for its normalization. The present study demonstrated the importance of using stable reference protein markers and normalization strategy in order to get correct results in semi-quantitative Western blot experiments in placental tissues.

Type de document: Article
Mots-clés libres: Amido black; Gestational diabetes mellitus; Normalization; Preeclampsia; Target protein; Western blot
Centre: Centre INRS-Institut Armand Frappier
Date de dépôt: 08 mars 2024 19:42
Dernière modification: 08 mars 2024 19:42
URI: https://espace.inrs.ca/id/eprint/14054

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