Dépôt numérique

Quantitative PCR Pitfalls: The Case of the Human Placenta

Lanoix, Dave; Lacasse, Andree-Anne; St-Pierre, Joey; Taylor, Sean C.; Ethier-Chiasson, Maude; Lafond, Julie et Vaillancourt, Cathy ORCID logoORCID: https://orcid.org/0000-0003-0543-6244 (2012). Quantitative PCR Pitfalls: The Case of the Human Placenta Molecular Biotechnology , vol. 52 , nº 3. pp. 234-243. DOI: 10.1007/s12033-012-9539-2.

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Reverse-transcription quant. polymerase chain reaction (RT-qPCR) is a rapid and high throughput gene expression quantification technol. In order to obtain accurate results, several key exptl. design and standardization steps must be rigorously followed as previously described in the Min. Information for Publication of Quant. Real-Time PCR Expts. (MIQE) guidelines. This study investigates the effect of ref. gene normalization and the impact of RNA degrdn. on gene expression of 8-oxoguanine DNA glycosylase in human placenta from pregnancies complicated by preeclampsia and gestational diabetes mellitus and their gestation-matched controls. The data presented here show how RNA quality and appropriate ref. gene selection is not only important to obtain accurate and reproducible RT-qPCR data but how different and even opposite results can be reported if the key steps outlined in the MIQE guidelines are not followed. The procedures and assocd. results presented in this study provide the first practical application of the MIQE guidelines to placental anal. in normal and pathol. pregnancies.

Type de document: Article
Mots-clés libres: -
Centre: Centre INRS-Institut Armand Frappier
Date de dépôt: 08 mars 2024 19:40
Dernière modification: 08 mars 2024 19:40
URI: https://espace.inrs.ca/id/eprint/14053

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