Impact on the endoplasmic reticulum and Golgi apparatus of turnip mosaic virus infection

Grangeon, Romain; Agbeci, Maxime; Chen, Jun; Grondin, Gilles; Zheng, Huanquan et Laliberté, Jean-Francois (2012). Impact on the endoplasmic reticulum and Golgi apparatus of turnip mosaic virus infection Journal of Virology , vol. 86 , nº 17. pp. 9255-9265. DOI: 10.1128/JVI.01146-12.

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Résumé

The impact of turnip mosaic virus (TuMV) infection on the endomembranes of the host early secretory pathway was investigated using an infectious clone that as been engineered for tagging viral membrane structures with a fluorescent protein fused to the viral protein 6K2. TuMV infection led to the amalgamation of the endoplasmic reticulum (ER), Golgi app., COPII coatamers, and chloroplasts into a perinuclear globular structure that also contained viral proteins. One consequence of TuMV infection was that protein secretion was blocked at the ER-Golgi interface. Fluorescence recovery after photobleaching (FRAP) expts. indicated that the perinuclear structure cannot be restocked in viral components but was dynamically connected to the bulk of the Golgi app. and the ER. Expts. with 6K2 fused to photoactivable green fluorescent protein (GFP) showed that prodn. of motile peripheral 6K2 vesicles was functionally linked to the perinuclear structure. Disruption of the early secretory pathway did not prevent the formation of the perinuclear globular structure, enhanced the clustering of peripheral 6K2 vesicles with COPII coatamers, and led to inhibition of cell-to-cell virus movement. This suggests that a functional secretory pathway is not required for the formation of the TuMV perinuclear globular structure and peripheral vesicles but is needed for successful viral intercellular propagation.

Type de document:	Article
Mots-clés libres:	-
Centre:	Centre INRS-Institut Armand Frappier
Date de dépôt:	08 mars 2024 20:04
Dernière modification:	08 mars 2024 20:04
URI:	https://espace.inrs.ca/id/eprint/14032

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