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In vitro effects of PCDDs/Fs on NK-like cell activity of Eisenia andrei earthworms


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Belmeskine, Hayet; Haddad, Sami; Vandelac, Louise et Fournier, Michel (2012). In vitro effects of PCDDs/Fs on NK-like cell activity of Eisenia andrei earthworms Journal of Xenobiotics , vol. 2 , nº 1. pp. 1-6. DOI: 10.4081/xeno.2012.e1.

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In this study, we assessed in vitro the effects of PCDD/Fs on the NK-like cell activity in Eisenia andrei earthworms using flow cytometry for anal. NK-like coelomocytes isolated from E. andrei and used as effectors were exposed to various concns. of PCDDs/Fs mixt., C1 (6.25 × 10-3 ng 2378-TCDD/mL), C2 (12.5 × 10-3 ng 2378-TCDD/mL) and C3 (25 × 10-3 ng 2378-TCDD/mL), before adding them to human tumoral cells (K562) used as targets. We evaluated the percentage of targets lysed by Nk-like cells. The results showed a significant stimulation of the NK-like activity at C3 when PCDD/Fs were not removed from effectors before contact with targets, while no effects were noted when the effectors were washed (PCDD/Fs removed) or fixed. Assessment of the viability of the targets (K562), exposed alone and sep. from effectors, to the three concns. of PCDD/Fs, C1, C2 and C3, showed that all these concns. were cytotoxic for K562. Results suggest that PCDD/Fs concns. tested in this assay may be considered too low to induce suppressive effects on the immune function such as the NK-like activity in E. andrei earthworms. [on SciFinder(R)]

Type de document: Article
Mots-clés libres: cytotoxicity perchlorodibenzodioxin perchlorodibenzofuran NK cell Eisenia
Centre: Centre INRS-Institut Armand Frappier
Date de dépôt: 06 mars 2024 15:08
Dernière modification: 06 mars 2024 15:08
URI: https://espace.inrs.ca/id/eprint/13987

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