Continuous Fixed-Bed Column Studies to Remove Polycyclic Aromatic Hydrocarbons by Degrading Enzymes Immobilized on Polyimide Aerogels.

Davoodi, Seyyed Mohammadreza; Miri, Saba; Brar, Satinder Kaur et Martel, Richard ORCID: https://orcid.org/0000-0003-4219-5582 (2023). Continuous Fixed-Bed Column Studies to Remove Polycyclic Aromatic Hydrocarbons by Degrading Enzymes Immobilized on Polyimide Aerogels. Journal of Water Process Engineering , vol. 53 , nº Juillet. p. 103597. DOI: 10.1016/j.jwpe.2023.103597.

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Résumé

The main sources of polycyclic aromatic hydrocarbons, entering aquatic environments are industrial discharges, petroleum spills, combustion of fossil fuels, urban runoff, and atmospheric deposition. For the biodegradation of polycyclic aromatic hydrocarbons, the use of biocatalysts, such as enzymes, is an environmentally friendly method. Despite this, it is necessary to immobilize the enzymes in order to facilitate their recovery and reusability, as well as to prevent their loss. Using covalent bonding, PAH degrading enzymes were immobilized on modified polyimide aerogels. Covalent immobilization of enzymes on modified polyimide aerogels resulted in around 9- and 6-fold lower enzyme leaching for naphthalene and catechol 2,3 dioxygenase enzymes compared to enzyme immobilization using adsorption. The Fourier Transform Infrared Spectrum (FTIR) confirmed the enzyme immobilization and aerogel modification. The effects of flow rate, size of aerogels and inlet concentration of anthracene on removal efficiency of pollutant were examined. Using the derived model as a basis for prediction, In terms of removal efficiency, the highest result was achieved to be 84.01% at flow rate of 22 ml min-1, initial concentration of 34 mg l-1 and aerogel size 2 cm while under these conditions, the removal efficiency was experimentally measured to be 87.14 %. Enzyme loaded-aerogel as a fixed-bed column for the removal of polycyclic aromatic hydrocarbons provides novel insight into the application of aerogel base materials for water treatment and PAH removal with possibilities of scaling up for larger applications.

Type de document:	Article
Mots-clés libres:	polycyclic aromatic hydrocarbons; enzymatic bioremediation; fixed-bed columns
Centre:	Centre Eau Terre Environnement
Date de dépôt:	02 nov. 2023 13:06
Dernière modification:	07 nov. 2023 14:29
URI:	https://espace.inrs.ca/id/eprint/13687

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